LOCUS       sequence-62887-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy28
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             123..131
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(247..270)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(297..319)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
       61 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgaatca
      121 ttacctcctt acgttacaag tataacacaa agtttttatg ttgtcaactg cagtttgggc
      181 tgcaggaatt cgatatcaag cttgtcgacg gagctcgaat tcggatccgg tacctcatta
      241 gtccatgggc ccctggaaca gaacttccag atccgatttt ggaggatggt cgccaccacc
      301 aaacgtggct tgccagccct gcaaaggcca tgctatatac ttgctggatt tcaagtactt
      361 atcaatttgt gggatagctt caatacgttt tttaaaacaa actaattttg ggaacgcatc
      421 caggcacatt gggtccatgt ataaaacaac atcaagagcg tcatacaaca tgaagtcagg
      481 atgggttaca tgatcaccat ttaaatatgt tttatgacat aaacgatctt cgaacatttt
      541 cagcatttca ggtagcttgc taagaaaatc aactttgaga gtttcaaagt ctttactata
      601 tgcaattctc gaaacaccgt atctaatatc caaaaccgct ccttcaagca ttgaaatctc
      661 tgcacgctct tttggacaac cacccaacat gttgtgcttg tcagctatat aacgtatgat
      721 ggccatagac tgtgttaatt taacatcacc atcaatataa taaggaagat tgggaaactc
      781 caaacccaat tcaaactttt tgtttcgcca tttatcacct tcatcgcgct catacaaatg
      841 ctcttca
//
