LOCUS       sequence-62888-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy29
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             123..131
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(247..270)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(283..306)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
       61 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgaatca
      121 ttacctcctt acgttacaag tataacacaa agtttttatg ttgtcaactg cagtttgggc
      181 tgcaggaatt cgatatcaag cttgtcgacg gagctcgaat tcggatccgg tacctcatta
      241 gtccatgggc ccctggaaca gaacttccag gctacccgga gtctgcgcgt ctttcagggc
      301 ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca
      361 gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatagt
      421 ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ctacggcacc
      481 cagcggtttg tctttattaa ccgcttccag accttcatca gtcagcagat agttttcgag
      541 gaactctttt gccagctctt tgttcggact ggcggcgtta atacctgcgc tcagcacgcc
      601 aacgaacggt ttggatggtt gacccttgaa ggtcggcagt accgttacac cataattcac
      661 tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc
      721 tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt
      781 aatcaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc
      841 tttaatgtcg tacttgccgt tttcatactt gaacgcataa cccccgtcag cagcaatcag
      901 cggccaggtg aagtac
//
