LOCUS       sequence-62889-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Okamoto K, Bartocci C, Ouzounov I, Diedrich JK, Yates Iii JR, Denchi
            EL
  TITLE     A two-step mechanism for TRF2-mediated chromosome-end protection.
  JOURNAL   Nature. 2013 Feb 6. doi: 10.1038/nature11873.
  PUBMED    23389450
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Feb 6. doi: 10.1038/nature11873."
COMMENT     Sequence Label: pLPC-Flag-TRF2(delta)iDDR
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tcagccacca tggactacaa
      121 agacgatgac gataaaggat ccatggcggg aggaggcggg agtagcgacg gcagcgggcg
      181 ggcagctggc aggcgggcgt cccgcagtag cgggcgggcc cggcgggggc gccacgagcc
      241 ggggctgggg ggcccggcgg agcgcggcgc gggggaggca cggctggaag aggcagtcaa
      301 tcgctgggtg ctcaagttct acttccacga ggcgctgcgg gcctttcggg gtagccggta
      361 cggggacttc agacagatcc gggacatcat gcaggctttg cttgtcaggc ccttggggaa
      421 ggagcacacc gtgtcccgat tgctgcgggt tatgcagtgt ctgtcgcgga ttgaagaagg
      481 ggaaaattta gactgttcct ttgatatgga ggctgagctc acaccactgg aatcagctat
      541 caatgtgctg gagatgatta aaacggaatt tacactgaca gaagcagtgg tcgaatccag
      601 tagaaaactg gtcaaggaag ctgctgtcat tatttgtatc aaaaacaaag aatttgaaaa
      661 ggcttcaaaa attttgaaaa aacatatgtc caaggacccc acaactcaga agctgagaaa
      721 tgatctcctg aatattattc gagaaaagaa cttggcccat cctgttatcc agaacttttc
      781 atatgagacc ttccagcaga agatgctgcg cttcctggag agccacctgg atgacgccga
      841 gccctacctc ctcacgatgg ccaaaaaggc tttgaaatct gagtccgctg cctcaagtac
      901 agggaaggaa gataaacagc cagcaccagg gcctgtggaa aa
//
