LOCUS       sequence-62890-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Okamoto K, Bartocci C, Ouzounov I, Diedrich JK, Yates Iii JR, Denchi
            EL
  TITLE     A two-step mechanism for TRF2-mediated chromosome-end protection.
  JOURNAL   Nature. 2013 Feb 6. doi: 10.1038/nature11873.
  PUBMED    23389450
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Feb 6. doi: 10.1038/nature11873."
COMMENT     Sequence Label: pLPC-Myc-TRFcT
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..142
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tcagccacca tggaacaaaa
      121 gttgatttct gaagaagatt tgttaagaaa gagaggatcc gcgggaggag gcgggagtag
      181 cgacggcagc gggcgggcag ctggcaggcg ggcgtcccgc agtagcgggc gggcccggcg
      241 ggggcgccac gagccggggc tggggggccc ggcggagcgc gaggaggagg aggaggacgc
      301 gggcctggtg gccgaggccg aggccgtggc tgccggctgg atgctcgatt tcctctgcct
      361 ctctctttgc cgagctttcc gcgacggccg ctccgaggac ttccgcagga cccgcaacag
      421 cgcagaggct attattcatg gactatccag tctaacagct tgccagttga gaacgatata
      481 catatgtcag tttttgacaa gaattgcagc aggaaaaacc cttgatgcac agtttgaaaa
      541 tgatgaacga attacaccct tggaatcagc cctgatgatt tggggttcaa ttgaaaagga
      601 acatgacaaa cttcatgaag aaatacagaa tttaattaaa attcaggcta tagctgtttg
      661 tatggaaaat ggcaacttta aagaagcaga agaagtcttt gaaagaatat ttggtgatcc
      721 aaattctcat atgcctttca aaagcaaatt gcttatgata atctctcaga aagatacatt
      781 tcattccttt tttcaacact tcagctacaa ccacatgatg gagaaaatta agagttatgt
      841 gaattatgtg ctaagtgaaa aatcatcaac ctttctaatg aaggcagcgg caaaagcttt
      901 aaaatctgag tccgctgcct caagtacagg gaaggaagat aaacagccag caccagggcc
      961 tgtggaaaag cc
//
