LOCUS       sequence-62905-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Pertz O, Hodgson L, Klemke RL, Hahn KM
  TITLE     Spatiotemporal dynamics of RhoA activity in migrating cells.
  JOURNAL   Nature. 2006 Apr 20. 440(7087):1069-72.
  PUBMED    16547516
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 20. 440(7087):1069-72."
COMMENT     Sequence Label: pTriEx-RhoA FLARE.sc Biosensor WT
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(347..598)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(389..410)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     738..757
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggatgatggg cacgttggga cagaaatgct tgacttctgg ggtccacttt tctgggatgt
       61 tttctaaact atcagggctg tcgatggaaa aacacatcag tataacatcg gtatctgggt
      121 aggagagggg cctcaggcga tcataatctt cctgcccagc tgtgtcccac aaagccaact
      181 ctacctgctt tccatccacc tcgatatctg ccacatagtt ctcaaacact gtgggcacat
      241 acacctctgg gaactggtcc ttgctgaaga ctatgagcaa gcatgtcttt ccacaggctc
      301 catcaccaac aatcaccagt ttcttccgga tggcagccat tccccccttg tacagctcgt
      361 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
      421 tctcgttggg gtctttgctc agggcggact ggtagctcag gtagtggttg tcgggcagca
      481 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
      541 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
      601 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
      661 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
      721 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
      781 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
      841 agcgggcgaa gcacatcagg ccgtagccga aggtggtcac gagggtgggc cagggcacgg
      901 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
      961 cgccctcgcc ggacacgctg aacttgtggc cgtttac
//
