LOCUS       sequence-62941-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALEN_v2 (NG)
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             386..691
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     570..589
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
       61 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
      121 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
      181 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
      241 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
      301 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
      361 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
      421 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
      481 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
      541 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
      601 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
      661 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      721 tgcaggtcga cggtctcgac tcacgcctga gcaggtagtg gctattgcat ccaacggagg
      781 gggcagaccc gcactggagt caatcgtggc ccagctttcg aggccggacc ccgcgctggc
      841 cgcactcact aatgatcatc ttgtagcgct ggcctgcctc ggcggacgac ccgccttgga
      901 tgcggtgaag aaggggctcc cgcacgcgcc tgcattgatt aagcggacca acagaaggat
      961 tcccgagagg ac
//
