LOCUS       sequence-62948-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALETF_v2 (NG)
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..85)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(107..256)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(281..301)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccccggtgaa cagctcctcg cccttgctca ctgggccagg attctcctcg acgtcaccgc
       61 atgttagcag acttcctctg ccctctccac tgcctctaga gttaatcagc atgtccaggt
      121 cgaaatcatc aagggcgtca ctgccgagca tgtcgaggtc aaagtcatca agggcatccg
      181 aaccaagcat gtcaaggtca aaatcatcga gggcgtcact tcccagcata tccagatcaa
      241 aatcgtccaa tgcgtcagcc cgtccggaac cgctggcctc cacctttctc ttcttcttgg
      301 ggctagctga cgcgcgagtt tggtcccctt catgcatggg cgagggcgcg tccaaatccc
      361 tctccagcga gtctgcgaac gcatgaaggc tcgcctggtc aggtgtctga gttgacgtag
      421 gtgaaggctt cgcgcgtttc ataccgctcg cttggagaat cctgtcccac cgctgggagg
      481 cgggaggcag tgtgcccgag cgggcctcga gttctgtgac accgactcta cgaaagagct
      541 gcagcagtcc gtgtctcgac ataccaaatt gagtcatggc gtcatcgaac gcttgtgcgg
      601 ggtgggagtg acactggaag aatccgagca cgcggaccac ttgcgcgtga tctgccactc
      661 gatgtgatgt cctctcggga atccttctgt tggtccgctt aatcaatgca ggcgcgtgcg
      721 ggagcccctt cttcaccgca tccaaggcgg gtcgtccgcc gaggcaggcc agcgctacaa
      781 gatgatcatt agtgagtgcg gccagcgcgg ggtccggcct cgaaagctgg gccacgattg
      841 actccagtgc gggtctgccc ccgccattgg atgcaatagc cactacctgc tcaggcgtga
      901 gtcgagaccg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattccc
//
