LOCUS       sequence-62953-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALETF_v2 (NG)
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             740..760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             785..934
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga cggtctcgac tcacgcctga gcaggtagtg gctattgcat ccaatggcgg
      181 gggcagaccc gcactggagt caatcgtggc ccagctttcg aggccggacc ccgcgctggc
      241 cgcactcact aatgatcatc ttgtagcgct ggcctgcctc ggcggacgac ccgccttgga
      301 tgcggtgaag aaggggctcc cgcacgcgcc tgcattgatt aagcggacca acagaaggat
      361 tcccgagagg acatcacatc gagtggcaga tcacgcgcaa gtggtccgcg tgctcggatt
      421 cttccagtgt cactcccacc ccgcacaagc gttcgatgac gccatgactc aatttggtat
      481 gtcgagacac ggactgctgc agctctttcg tagagtcggt gtcacagaac tcgaggcccg
      541 ctcgggcaca ctgcctcccg cctcccagcg gtgggacagg attctccaag cgagcggtat
      601 gaaacgcgcg aagccttcac ctacgtcaac tcagacacct gaccaggcga gccttcatgc
      661 gttcgcagac tcgctggaga gggatttgga cgcgccctcg cccatgcatg aaggggacca
      721 aactcgcgcg tcagctagcc ccaagaagaa gagaaaggtg gaggccagcg gttccggacg
      781 ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc tcgatgattt
      841 tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca tgctcggcag
      901 tgacgccctt gatgatttcg acctggacat gctgattaac tctagaggca
//
