LOCUS       sequence-62960-e        9865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Liu D, Davydenko O, Lampson MA
  TITLE     Polo-like kinase-1 regulates kinetochore-microtubule dynamics and 
            spindle checkpoint silencing.
  JOURNAL   J Cell Biol. 2012 Aug 20;198(4):491-9. doi: 10.1083/jcb.201205090.
  PUBMED    22908307
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2012-08-20- 20"; volume: "198"; 
            issue: "4"; pages: "491-9"
COMMENT     Sequence Label: Hec1 mCherry Plk1 T210D
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2850..2859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2856..3563
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3001..3019)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3269..3288
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5421..5439)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5422..5439)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5459..5476)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5465..5689
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5735..6163
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5822..5841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6032..6053
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6172..6192)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6177..6506
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6357..6492
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6419..6438
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6573..7367
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6627..6646)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7237..7256
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7541..7662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7578..7597)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7632..7651
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7711..7727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7711..7727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7724..7746)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7735..7751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7759..7765,7766..7783,7784..7789))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7804..7825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7942..7959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8113..8698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8193..8212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8869..9660,9661..9729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9492..9511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9730..9834)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccgccaccat gaagcgcagt tcagtttcca gcggtggtgc tggccgcctc tccatgcagg
      961 agttaagatc ccaggatgta aataaacaag gcctctatac ccctcaaacc aaagagaaac
     1021 caacctttgg aaagttgagt ataaacaaac cgacatctga aagaaaagtc tcgctatttg
     1081 gcaaaagaac tagtggacat ggatcccgga atagtcaact tggtatattt tccagttctg
     1141 agaaaatcaa ggacccgaga ccacttaatg acaaagcatt cattcagcag tgtattcgac
     1201 aactctgtga gtttcttaca gaaaatggtt atgcacataa tgtgtccatg aaatctctac
     1261 aagctccctc tgttaaagac ttcctgaaga tcttcacatt tctttatggc ttcctgtgcc
     1321 cctcatacga acttcctgac acaaagtttg aagaagaggt tccaagaatc tttaaagacc
     1381 ttgggtatcc ttttgcacta tccaaaagct ccatgtacac agtgggggct cctcatacat
     1441 ggcctcacat tgtggcagcc ttagtttggc taatagactg catcaagata catactgcca
     1501 tgaaagaaag ctcaccttta tttgatgatg ggcagccttg gggagaagaa actgaagatg
     1561 gaattatgca taataagttg tttttggact acaccataaa atgctatgag agttttatga
     1621 gtggtgccga cagctttgat gagatgaatg cagagctgca gtcaaaactg aaggatttat
     1681 ttaatgtgga tgcttttaag ctggaatcat tagaagcaaa aaacagagca ttgaatgaac
     1741 agattgcaag attggaacaa gaaagagaaa aagaaccgaa tcgtctagag tcgttgagaa
     1801 aactgaaggc ttccttacaa ggagatgttc aaaagtatca ggcatacatg agcaatttgg
     1861 agtctcattc agccattctt gaccagaaat taaatggtct caatgaggaa attgctagag
     1921 tagaactaga atgtgaaaca ataaaacagg agaacactcg actacagaat atcattgaca
     1981 accagaagta ctcagttgca gacattgagc gaataaatca tgaaagaaat gaattgcagc
     2041 agactattaa taaattaacc aaggacctgg aagctgaaca acagaagttg tggaatgagg
     2101 agttaaaata tgccagaggc aaagaagcga ttgaaacaca attagcagag tatcacaaat
     2161 tggctagaaa attaaaactt attcctaaag gtgctgagaa ttccaaaggt tatgactttg
     2221 aaattaagtt taatcccgag gctggtgcca actgccttgt caaatacagg gctcaagttt
     2281 atgtacctct taaggaactc ctgaatgaaa ctgaagaaga aattaataaa gccctaaata
     2341 aaaaaatggg tttggaggat actttagaac aattgaatgc aatgataaca gaaagcaaga
     2401 gaagtgtgag aactctgaaa gaagaagttc aaaagctgga tgatctttac caacaaaaaa
     2461 ttaaggaagc agaggaagag gatgaaaaat gtgccagtga gcttgagtcc ttggagaaac
     2521 acaagcacct gctagaaagt actgttaacc aggggctcag tgaagctatg aatgaattag
     2581 atgctgttca gcgggaatac caactagttg tgcaaaccac gactgaagaa agacgaaaag
     2641 tgggaaataa cttgcaacgt ctgttagaga tggttgctac acatgttggg tctgtagaga
     2701 aacatcttga ggagcagatt gctaaagttg atagagaata tgaagaatgc atgtcagaag
     2761 atctctcgga aaatattaaa gagattagag ataagtatga gaagaaagct actctaatta
     2821 agtcttctga agaagagcta gcatgcttcg ccaccatggt gagcaagggc gaggaggata
     2881 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2941 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3001 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     3061 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     3121 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     3181 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     3241 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     3301 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3361 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3421 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3481 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3541 gcggcatgga cgagctgtac aagagatctc gagctcaagc ttcgaattcg atgagtgctg
     3601 cagtgactgc agggaagctg gcacgggcac cggccgaccc tgggaaagcc ggggtccccg
     3661 gagttgcagc tcccggagct ccggcggcgg ctccaccggc gaaagagatc ccggaggtcc
     3721 tagtggaccc acgcagccgg cggcgctatg tgcggggccg ctttttgggc aagggcggct
     3781 ttgccaagtg cttcgagatc tcggacgcgg acaccaagga ggtgttcgcg ggcaagattg
     3841 tgcctaagtc tctgctgctc aagccgcacc agagggagaa gatgtccatg gaaatatcca
     3901 ttcaccgcag cctcgcccac cagcacgtcg taggattcca cggctttttc gaggacaacg
     3961 acttcgtgtt cgtggtgttg gagctctgcc gccggaggtc tctcctggag ctgcacaaga
     4021 ggaggaaagc cctgactgag cctgaggccc gatactacct acggcaaatt gtgcttggct
     4081 gccagtacct gcaccgaaac cgagttattc atcgagacct caagctgggc aaccttttcc
     4141 tgaatgaaga tctggaggtg aaaatagggg attttggact ggcaaccaaa gtcgaatatg
     4201 acggggagag gaagaaggac ctgtgtggga ctcctaatta catagctccc gaggtgctga
     4261 gcaagaaagg gcacagtttc gaggtggatg tgtggtccat tgggtgtatc atgtatacct
     4321 tgttagtggg caaaccacct tttgagactt cttgcctaaa agagacctac ctccggatca
     4381 agaagaatga atacagtatt cccaagcaca tcaaccccgt ggccgcctcc ctcatccaga
     4441 agatgcttca gacagatccc actgcccgcc caaccattaa cgagctgctt aatgacgagt
     4501 tctttacttc tggctatatc cctgcccgtc tccccatcac ctgcctgacc attccaccaa
     4561 ggttttcgat tgctcccagc agcctggacc ccagcaaccg gaagcccctc acagtcctca
     4621 ataaaggctt ggagaacccc ctgcctgagc gtccccggga aaaagaagaa ccagtggttc
     4681 gagagacagg tgaggtggtc gactgccacc tcagtgacat gctgcagcag ctgcacagtg
     4741 tcaatgcctc caagccctcg gagcgtgggc tggtcaggca agaggaggct gaggatcctg
     4801 cctgcatccc catcttctgg gtcagcaagt gggtggacta ttcggacaag tacggccttg
     4861 ggtatcagct ctgtgataac agcgtggggg tgctcttcaa tgactcaaca cgcctcatcc
     4921 tctacaatga tggtgacagc ctgcagtaca tagagcgtga cggcactgag tcctacctca
     4981 ccgtgagttc ccatcccaac tccttgatga agaagatcac cctccttaaa tatttccgca
     5041 attacatgag cgagcacttg ctgaaggcag gtgccaacat cacgccgcgc gaaggtgatg
     5101 agctcgcccg gctgccctac ctacggacct ggttccgcac ccgcagcgcc atcatcctgc
     5161 acctcagcaa cggcagcgtg cagatcaact tcttccagga tcacaccaag ctcatcttgt
     5221 gcccactgat ggcagccgtg acctacatcg acgagaagcg ggacttccgc acataccgcc
     5281 tgagtctcct ggaggagtac ggctgctgca aggagctggc cagccggctc cgctacgccc
     5341 gcactatggt ggacaagctg ctgagctcac gctcggccag caaccgtctc aaggcctcct
     5401 aagaatctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     5461 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5521 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5581 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5641 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5701 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     6181 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6241 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6301 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6361 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6421 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     6481 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     6541 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     7381 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     7441 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7501 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7621 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7681 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7741 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7801 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7861 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7921 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7981 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8041 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8281 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8341 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8401 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8461 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8521 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8581 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8641 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8761 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8821 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8881 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8941 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9001 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9061 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9121 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9181 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9241 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9301 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9361 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9421 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9481 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9541 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9601 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9661 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9721 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9781 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9841 tccccgaaaa gtgccacctg acgtc
//
