LOCUS       sequence-62966-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Anthouard R, DiRita VJ
  TITLE     Small-molecule inhibitors of toxT expression in Vibrio cholerae.
  JOURNAL   MBio. 2013 Aug 6;4(4). pii: e00403-13. doi: 10.1128/mBio.00403-13.
  PUBMED    23919997
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2013-08-6"; volume: "4(4). pii: e00403-13. doi"; pages: " 
            10.1128/mBio.00403-13"
COMMENT     Sequence Label: pMMB66EH-aphA
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(555..577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    566..582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(590..596,597..612,613..618))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cttttatgcc atcgcgttca attctgccaa cacttcgtcc gcccattgaa tccatgcttg
       61 gcgaaccagt aaattacgac gtagcgttaa acgctctaaa cgttgctgct tgtctaacac
      121 ggctgggtta gcgtaataag cggcttcgat ttcctgatag tgagcgacca atttacgcga
      181 ttcttcaacc agttcagcca gttgcagacg gtaaggttct gccgattgta ctgaacaagc
      241 catcaatttc gcactaaact catcacgcac ggttgggtgt gcggttggtt gatcaaacca
      301 ctcacctaaa gcgctgcggc cagcttgggt tatagaataa accttgcgat ctggtttgcc
      361 ttcctgtggt tccagaacac aagtcactaa accctgttcc cccattttat tgagttcacg
      421 atatacctgc tgatggctgg ctttccagaa gtagccaatg ctagcagaaa actctttggt
      481 tatatcgtac ccggttgcat cgcgtgtgct aagaacagta aggataacgt gtggtaatga
      541 catgaattct gtttcctgtg tgaaattgtt atccgctcac aattccacac attatacgag
      601 ccgatgatta attgtcaaca gctcatttca gaatatttgc cagaaccgtt atgatgtcgg
      661 cgcaaaaaac attatccaga acgggagtgc gccttgagcg acacgaatta tgcagtgatt
      721 tacgacctgc acagccatac cacagcttcc gatggctgcc tgacgccaga agcattggtg
      781 caccgtgcag tcgataagcc cggatcagct tgcaattc
//
