LOCUS       sequence-62971-e        9289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, 
            Lampson MA
  TITLE     Regulated targeting of protein phosphatase 1 to the outer 
            kinetochore by KNL1 opposes Aurora B kinase.
  JOURNAL   J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. 
            Epub 2010 Mar 15.
  PUBMED    20231380
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201001006"; journalName: "J Cell Biol"; date: 
            "2010-03-22- 22"; volume: "188"; issue: "6"; pages: "809-20"
COMMENT     Sequence Label: C-term Hec1-targeted Aurora B FRET sensor
FEATURES             Location/Qualifiers
     source          1..9289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2841..2850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2847..3554
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4113..4115,4116..4829)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     primer_bind     complement(4155..4176)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4416..4435)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(4845..4863)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4846..4863)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4883..4900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4889..5113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5159..5587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5246..5265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5456..5477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5596..5616)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5601..5930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5781..5916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5843..5862
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5997..6791
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6051..6070)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6661..6680
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6965..7086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7002..7021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7056..7075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7135..7151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7135..7151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7148..7170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7159..7175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7183..7189,7190..7207,7208..7213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7228..7249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7366..7383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7537..8122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7617..7636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8293..9084,9085..9153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8916..8935
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9154..9258)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcgcg
      901 ccatgaagcg cagttcagtt tccagcggtg gtgctggccg cctctccatg caggagttaa
      961 gatcccagga tgtaaataaa caaggcctct atacccctca aaccaaagag aaaccaacct
     1021 ttggaaagtt gagtataaac aaaccgacat ctgaaagaaa agtctcgcta tttggcaaaa
     1081 gaactagtgg acatggatcc cggaatagtc aacttggtat attttccagt tctgagaaaa
     1141 tcaaggaccc gagaccactt aatgacaaag cattcattca gcagtgtatt cgacaactct
     1201 gtgagtttct tacagaaaat ggttatgcac ataatgtgtc catgaaatct ctacaagctc
     1261 cctctgttaa agacttcctg aagatcttca catttcttta tggcttcctg tgcccctcat
     1321 acgaacttcc tgacacaaag tttgaagaag aggttccaag aatctttaaa gaccttgggt
     1381 atccttttgc actatccaaa agctccatgt acacagtggg ggctcctcat acatggcctc
     1441 acattgtggc agccttagtt tggctaatag actgcatcaa gatacatact gccatgaaag
     1501 aaagctcacc tttatttgat gatgggcagc cttggggaga agaaactgaa gatggaatta
     1561 tgcataataa gttgtttttg gactacacca taaaatgcta tgagagtttt atgagtggtg
     1621 ccgacagctt tgatgagatg aatgcagagc tgcagtcaaa actgaaggat ttatttaatg
     1681 tggatgcttt taagctggaa tcattagaag caaaaaacag agcattgaat gaacagattg
     1741 caagattgga acaagaaaga gaaaaagaac cgaatcgtct agagtcgttg agaaaactga
     1801 aggcttcctt acaaggagat gttcaaaagt atcaggcata catgagcaat ttggagtctc
     1861 attcagccat tcttgaccag aaattaaatg gtctcaatga ggaaattgct agagtagaac
     1921 tagaatgtga aacaataaaa caggagaaca ctcgactaca gaatatcatt gacaaccaga
     1981 agtactcagt tgcagacatt gagcgaataa atcatgaaag aaatgaattg cagcagacta
     2041 ttaataaatt aaccaaggac ctggaagctg aacaacagaa gttgtggaat gaggagttaa
     2101 aatatgccag aggcaaagaa gcgattgaaa cacaattagc agagtatcac aaattggcta
     2161 gaaaattaaa acttattcct aaaggtgctg agaattccaa aggttatgac tttgaaatta
     2221 agtttaatcc cgaggctggt gccaactgcc ttgtcaaata cagggctcaa gtttatgtac
     2281 ctcttaagga actcctgaat gaaactgaag aagaaattaa taaagcccta aataaaaaaa
     2341 tgggtttgga ggatacttta gaacaattga atgcaatgat aacagaaagc aagagaagtg
     2401 tgagaactct gaaagaagaa gttcaaaagc tggatgatct ttaccaacaa aaaattaagg
     2461 aagcagagga agaggatgaa aaatgtgcca gtgagcttga gtccttggag aaacacaagc
     2521 acctgctaga aagtactgtt aaccaggggc tcagtgaagc tatgaatgaa ttagatgctg
     2581 ttcagcggga ataccaacta gttgtgcaaa ccacgactga agaaagacga aaagtgggaa
     2641 ataacttgca acgtctgtta gagatggttg ctacacatgt tgggtctgta gagaaacatc
     2701 ttgaggagca gattgctaaa gttgatagag aatatgaaga atgcatgtca gaagatctct
     2761 cggaaaatat taaagagatt agagataagt atgagaagaa agctactcta attaagtctt
     2821 ctgaagagct agcctcgagc gccaccatgg tgagcaaggg cgaggagacc acaatgggcg
     2881 taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct
     2941 tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg
     3001 aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct
     3061 acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct
     3121 tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg
     3181 tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg
     3241 agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca
     3301 ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc
     3361 tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg
     3421 tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg
     3481 actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg
     3541 acgagctgta caaggctggt accaagggta atggtaggtt tttaacttta aaaccattgc
     3601 ctgacagcat tattcaagaa agcctggaga ttcagcaagg tgtgaatcca tttttcattg
     3661 gtagatccga ggattgcaat tgtaaaattg aagacagtag gttgtctcga gttcattgct
     3721 tcattttcaa aaagaggcat gctgtaggca aaagcatgta tgaatctccg gcacaaggtt
     3781 tagatgatat ttggtattgc cacaccggaa ctaacgtgag ctatttaaat aataaccgca
     3841 tgatacaggg tacgaaattc cttttacaag acggagatga aatcaagatc atttgggata
     3901 aaaacaataa atttgtcatt ggctttaaag tggaaattaa cgatactaca ggtctgttta
     3961 acgagggatt aggtatgtta caagaacaaa gagtagtact taagcaaaca gccgaagaaa
     4021 aagatttggt gaaaaagtta ggaggatccg gcggcaaggt gaacaagatc gtgaagaacc
     4081 gccgcaccgt ggccatcggt ggttccggag gagtgagcaa gggcgaggag ctgttcaccg
     4141 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4201 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg ctctgcacca
     4261 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt
     4321 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4381 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4441 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4501 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4561 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     4621 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4681 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgttcaaag
     4741 accccaacga gaagcgcgat cacatggtcc tgctggagtt cctgaccgcc gccgggatca
     4801 ctgaaggcat gaacgagctg tacaagtaat ctagagggcc ctattctata gtgtcaccta
     4861 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4921 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4981 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5041 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5101 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     5161 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5221 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5281 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5341 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5401 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5461 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5521 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5581 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5701 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5881 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5941 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     6001 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     6061 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     6121 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     6181 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     6241 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     6301 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     6361 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     6421 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     6481 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     6541 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6601 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6661 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6721 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     6781 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     6841 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6901 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6961 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7021 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7081 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7141 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7201 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7261 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7321 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7381 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8041 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     8101 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8161 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8221 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8281 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8341 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8401 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8461 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8521 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8581 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8641 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8701 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8761 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8821 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8881 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8941 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9001 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9061 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9121 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9181 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9241 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
