LOCUS       sequence-62973-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALEN_v2 (NI)
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             162..182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        join(651..656,657..674,675..681)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(801..820)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg gccaccatgg actataagga ccacgacgga gactacaagg
      121 atcatgatat tgattacaaa gacgatgacg ataagatggc cccaaagaag aagcggaagg
      181 tcggtatcca cggagtccca gcagccgtag atttgagaac tttgggatat tcacagcagc
      241 agcaggaaaa gatcaagccc aaagtgaggt cgacagtcgc gcagcatcac gaagcgctgg
      301 tgggtcatgg gtttacacat gcccacatcg tagccttgtc gcagcaccct gcagcccttg
      361 gcacggtcgc cgtcaagtac caggacatga ttgcggcgtt gccggaagcc acacatgagg
      421 cgatcgtcgg tgtggggaaa cagtggagcg gagcccgagc gcttgaggcc ctgttgacgg
      481 tcgcgggaga gctgagaggg cctccccttc agctggacac gggccagttg ctgaagatcg
      541 cgaagcgggg aggagtcacg gcggtcgagg cggtgcacgc gtggcgcaat gcgctcacgg
      601 gagcacccct caacctgaca gagaccgcgg ccgcattagg caccccaggc tttacacttt
      661 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
      721 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      781 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      841 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      901 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgta
//
