LOCUS       sequence-62977-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALEN_v2 (NI)
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             388..693
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     572..591
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg
       61 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
      121 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
      181 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
      241 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
      301 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
      361 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
      421 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
      481 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
      541 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
      601 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
      661 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
      721 tctgcaggtc gacggtctcg actcacgcct gagcaggtag tggctattgc atccaacatc
      781 gggggcagac ccgcactgga gtcaatcgtg gcccagcttt cgaggccgga ccccgcgctg
      841 gccgcactca ctaatgatca tcttgtagcg ctggcctgcc tcggcggacg acccgccttg
      901 gatgcggtga agaaggggct cccgcacgcg cctgcattga ttaagcggac caacagaagg
      961 attcccgaga ggacat
//
