LOCUS       sequence-63-expo        5297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD57_56
FEATURES             Location/Qualifiers
     source          1..5297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2871..2893)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2993..3012
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3090..3108)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3176..3280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3281..3349,3350..4141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3499..3518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4312..4900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4801..4820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5054..5071
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5188..5209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5224..5229,5230..5247,5248..5254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5262..5278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5267..5289
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(5286..5297,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(5286..5297,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtacc cggagtagtt ggcgaagata
       61 gaaatggtgt tatcttatat aaattcagac tttctctgtt aaaattttaa ccaccaaaaa
      121 atatagaatt tataaaaagt ttcattttga aattttatct tttggggcca tttcactacc
      181 ttatcaggaa gaaatgtgtt tcatcagaaa ttgaattttt catcttttca gaaagtgtac
      241 ctaataccgt atcatgctca tattcacgtg aataaggctt ctgcccagtt ctgtgattca
      301 aggaaaacta cgaaaaatga tctcattttc tttgatttat caccgagctg gtgtgtcatc
      361 acacacagtc tgtttcttga taaaaacgcg cagaagttag agcattcatt gtgattttgg
      421 gtctttaatt tctgtaaaac aaataaagtg acaagtttat catctgccta ttttatcagt
      481 tatatgcatg gttagttgca aataagggag tagaaattga ctaaaactca aaatgagcac
      541 tgaaacgagg ggttttgtta gttgcgaaat aacaattaat acaatctggt tgattacttt
      601 gtaaaaggat aataaaatat gcaaaacttg gcgtattctg agatttcatt cctaaagaca
      661 tttatcaaaa caaatgtttt ctgcaatttt ctaaattctt ggaattctgc ccagcagcct
      721 ctaatatcgc gttttactta gaaaatgact tttggtttca ttttctcatt tctggcttct
      781 atttttcaca aatgcaaata ttacatttaa attagtactt tcatttctac aaagatcaaa
      841 tttaacatta ttgacaggtt tactgttatc ttgtgacagt tccgtactgt caatcaaaac
      901 gaaaattaaa actcatgaaa tatcaatgaa cataaattga aaaaaaaacc cagttgaaag
      961 gagaaaataa gcgaaacaca ccaacaactg agtagttacg ctcacaattt ggaaatgatt
     1021 ttgaaaactc tctggactgc atttcaattt ttaacaaaca atgaatacat actgatagtt
     1081 tttgtacttc tcggcattca gaggcaactt cctgccaaca acctggcaac ttatttagtg
     1141 ccactaaaat aagttgccaa attgttggca ggaagttgcc tccaaatgcc gagttggagc
     1201 aaatgattcg atatgatttt aaggatacct acgcatgcct cctgccaaga cacgcaccca
     1261 cctactgaaa tgtcgcggaa ttttacctcc caaactatag attgggtgaa acttcactgt
     1321 aaaagctttt tttaagtagt aggctggcag taatgaagac gtccatacca taatttttaa
     1381 tgttgtatag aaaatctgaa ttctcaagta taacattttc caataattag catagtcaga
     1441 aactctccac cgccgttccg ttcctttttt ttgtaactat ggagtaatag aacaagatta
     1501 ttcaaattca aatttttata aaactttaca acgtcctttc catatattct tcacacaaca
     1561 tttgaaaaaa caacaaattc attcgaaatg cattgcccat aatgaatcga ccgaaaaaca
     1621 caagtgaccg tcaggagatc gatagagaga gcccgttcca actagacaac tttttagtgc
     1681 ttatccttac acacactttc tagcttcata agaaatgcat ctattatcgt caccatttgg
     1741 agacaccagt tttagcgcac attaataatc gatcagggtc tagagactcc tgttggattg
     1801 gcatgccacg cctacatgac atctggctct tgacgggtga tttgttacag tgagtagcca
     1861 caatttcgcg cattttcttc taaatttcac tgttatttgt tcttctgatt tatttcattt
     1921 gaactacgga aatcggatcc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga
     1981 tactaacata acatagaaca ttttcaggag gacccttggc tagcgtcgac ggtaccatgg
     2041 tattgatatc tgagctccgc atcggccgct gtcatcagat cgccatctcg cgcccgtgcc
     2101 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     2161 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     2221 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     2281 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     2341 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     2401 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     2461 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     2521 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     2581 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     2641 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     2701 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     2761 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
     2821 cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2881 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2941 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     3001 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     3061 agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa
     3121 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3181 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3241 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3301 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3361 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3421 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3481 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3541 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3601 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3661 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3721 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3781 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3841 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3901 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3961 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4021 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4081 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4141 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4201 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4261 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4321 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4381 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4441 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4501 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4561 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4621 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4681 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     4741 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4861 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4921 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4981 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5041 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5101 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5161 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5221 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5281 tcacacagga aacagct
//
