LOCUS       sequence-63034-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Chubiz LM, Lee MC, Delaney NF, Marx CJ
  TITLE     FREQ-Seq: a rapid, cost-effective, sequencing-based method to 
            determine allele frequencies directly from mixed populations.
  JOURNAL   PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub
            2012 Oct 31.
  PUBMED    23118913
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47959. doi"; pages: " 
            10.1371/journal.pone.0047959. Epub 2012 Oct 31"
COMMENT     Sequence Label: pFSBC15
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     198..215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     399..415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(461..480)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     580..602
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(640..658)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
       61 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      241 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      301 actggaaagc gggcagtgag cgcaacgcaa ttaaaatgat acggcgacca ccgagatcta
      361 cactctttcc ctacacgacg ctcttccgat ctagtgaggt aaaacgacgg ccagttacgc
      421 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      481 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      541 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
      601 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
      661 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      781 tgagacaata accct
//
