LOCUS       sequence-63036-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Chubiz LM, Lee MC, Delaney NF, Marx CJ
  TITLE     FREQ-Seq: a rapid, cost-effective, sequencing-based method to 
            determine allele frequencies directly from mixed populations.
  JOURNAL   PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub
            2012 Oct 31.
  PUBMED    23118913
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47959. doi"; pages: " 
            10.1371/journal.pone.0047959. Epub 2012 Oct 31"
COMMENT     Sequence Label: pFSBC31
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     196..213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     397..413
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(459..478)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     578..600
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(638..656)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
       61 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      241 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      301 tggaaagcgg gcagtgagcg caacgcaatt aaaatgatac ggcgaccacc gagatctaca
      361 ctctttccct acacgacgct cttccgatct tagccagtaa aacgacggcc agttacgcat
      421 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      481 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      541 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      601 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
      661 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      721 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      781 agacaataac catgataaat gcttcaataa tattgaaaaa
//
