LOCUS       sequence-63048-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Chubiz LM, Lee MC, Delaney NF, Marx CJ
  TITLE     FREQ-Seq: a rapid, cost-effective, sequencing-based method to 
            determine allele frequencies directly from mixed populations.
  JOURNAL   PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub
            2012 Oct 31.
  PUBMED    23118913
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47959. doi"; pages: " 
            10.1371/journal.pone.0047959. Epub 2012 Oct 31"
COMMENT     Sequence Label: pFSBC48
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     193..210
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     394..410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(456..475)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     575..597
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(635..653)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
       61 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      121 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      181 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      241 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      301 aaagcgggca gtgagcgcaa cgcaattaaa atgatacggc gaccaccgag atctacactc
      361 tttccctaca cgacgctctt ccgatctgta caagtaaaac gacggccagt tacgcatctg
      421 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
      481 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      541 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      601 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
      661 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      721 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcat
//
