LOCUS       sequence-63054-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     pETCON Yeast Surface Display Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pETcon(-)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     93..116
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             448..459
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             460..486
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             602..631
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc
       61 aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg
      121 agaaaaaacc ccggatcgaa ttccctactt catacatttt caattaagat gcagttactt
      181 cgctgttttt caatattttc tgttattgct tcagttttag cacaggaact gacaactata
      241 tgcgagcaaa tcccctcacc aactttagaa tcgacgccgt actctttgtc aacgactact
      301 attttggcca acgggaaggc aatgcaagga gtttttgaat attacaaatc agtaacgttt
      361 gtcagtaatt gcggttctca cccctcaaca actagcaaag gcagccccat aaacacacag
      421 tatgttttta aggacaatag ctcgacgatt gaaggtagat acccatacga cgttccagac
      481 tacgctctgc aggctagtgg tggaggaggc tctggtggag gcggtagcgg aggcggaggg
      541 tcggctagcc atatgtgaag catgcttaat taaggcgcct tatctcgagg ggggcggatc
      601 cgaacaaaag cttatttctg aagaggactt gtaatagaga tctgataaca acagtgtaga
      661 tgtaacaaaa tcgactttgt tcccactgta cttttagctc gtacaaaata caatatactt
      721 ttcatttctc cgtaaacaac atgttttccc atgtaatatc cttttctatt tttcgttccg
      781 ttaccaactt tacacatact ttatatagct attcacttct atacactaaa aaactaagac
      841 aattttaatt ttgctgcctg ccatatttca atttg
//
