LOCUS       sequence-63056-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgc caccatgggt gactggagtg ccttggggaa
      121 attactggac aaggtccaag cctactccac cgctggaggg aaggtgtggc tgtcagtgct
      181 cttcatattc agaatcctgc tcctggggac agctgttgag tcagcttggg gtgatgaaca
      241 gtctgccttt cgctgtaaca ctcaacaacc tggctgcgaa aacgtctgct atgacaagtc
      301 cttccccatc tctcacgtgc gcttctgggt ccttcagatc atattcgtgt ctgtgcccac
      361 actcctgtac ttggcccatg tgttctatgt gatgaggaag gaagagaagc taaacaagaa
      421 agaagaggag ctcaaagtgg cccagactga cggggtcaac gtggagatgc acctgaagca
      481 gattgaaatc aagaagttca agtacgggat tgaagagcac ggcaaggtga aaatgagggg
      541 cggcttgctg agaacctaca tcatcagcat cctcttcaag tctgtcttcg aggtggcctt
      601 cctgctcatc cagtggtaca tctatgggtt cagcttgagc gcggtctaca cctgcaagag
      661 agatccctgc ccccaccagg tagactgctt cctctcacgt cccacggaga aaaccatctt
      721 catcatcttc atgctggtgg tgtccttggt gtctctcgct ttgaacatca ttgagctctt
      781 ctacgtcttc ttcaagggcg tt
//
