LOCUS       sequence-63103-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X
  TITLE     Generation of gene-modified mice via Cas9/RNA-mediated gene 
            targeting.
  JOURNAL   Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46.
  PUBMED    23545779
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Apr 2. doi: 10.1038/cr.2013.46."
COMMENT     Sequence Label: pST1374-NLS-flag-linker-Cas9
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaagggcc cctagtcgcc gccgagctga gacaggtcga tgcgagtctc
      121 gtacagtccg gtaattgact ggtgtatcag ggtggcatcg agaacttctt tggtagaggt
      181 gtaccgcttc ctgtcaatag ttgtatcgaa atacttgaag gcagcaggag cgcccagatt
      241 agtcagagta aagaggtgga taatattctc tgcttgttcg cgaattggct tgtccctgtg
      301 cttattatat gcgctcagca ctttatcgag gtttgcatcg gccagaataa cccgcttgct
      361 gaactcgcta atctgttcaa tgatttcgtc caggtagtgt ttatgttgct caacaaagag
      421 ttgcttctgc tcattgtctt cagggctacc tttgagtttc tcgtagtggg aggccagata
      481 caggaagttc acgtatttgg agggcagagc cagctcgttt cctttctgca gctctccggc
      541 ggaggccagc atccgcttcc taccattctc cagctcaaag agagagtact tgggcagttt
      601 gatgatgaga tctttcttca cttctttata gcccttagct tccaggaaat cgattggatt
      661 cttctcgaag ctggatctct ccataatagt aattccgagc agctccttaa cagacttgag
      721 tttcttggac ttgcctttct ccacttttgc cacgaccaga acggaataag ccactgtagg
      781 ggaatcgaaa ccgccatact tctttgggtc ccaatctttc ttcctggcga tcagcttgtc
      841 agagttccgc tttggcagga tgctctcctt tgagaatccg ccggtctgca cttcggtctt
      901 cttcacgata ttgacttgtg gcatggacag caccttccgc acagttgcga agtccctgcc
      961 tttatcccac acgatttctc ctgtttctcc gtttgtttcg atca
//
