LOCUS       sequence-63111-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Gunn A, Bennardo N, Cheng A, Stark JM
  TITLE     Correct end use during end joining of multiple chromosomal double 
            strand breaks is influenced by repair protein RAD50, DNA-dependent 
            protein kinase DNA-PKcs, and transcription context.
  JOURNAL   J Biol Chem. 2011 Dec 9;286(49):42470-82. doi: 
            10.1074/jbc.M111.309252. Epub 2011 Oct 24.
  PUBMED    22027841
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.309252"; journalName: "J Biol Chem"; date: 
            "2011-12-9- 9"; volume: "286"; issue: "49"; pages: "42470-82"
COMMENT     Sequence Label: pCAGGS-I-SceI-Trex2
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             125..145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             167..187
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             206..232
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
       61 tctcatcatt ttggcaaaga attcctcgaa attcagatcc gccgccacta tgggatcaag
      121 atcgccaaaa aagaagagaa aggtgccgaa gaagcatgca gcaccaccaa aaaaaaaacg
      181 aaaagtagaa gacccacgat ttatgtaccc atacgatgtt cctgactatg cgggtatgaa
      241 aaacatcaaa aaaaaccagg taatgaacct gggtccgaac tctaaactgc tgaaagaata
      301 caaatcccag ctgatcgaac tgaacatcga acagttcgaa gcaggtatcg gtctgatcct
      361 gggtgatgct tacatccgtt ctcgtgatga aggtaaaacc tactgtatgc agttcgagtg
      421 gaaaaacaaa gcatacatgg accacgtatg tctgctgtac gatcagtggg tactgtcccc
      481 gccgcacaaa aaagaacgtg ttaaccacct gggtaacctg gtaatcacct ggggcgccca
      541 gactttcaaa caccaagctt tcaacaaact ggctaacctg ttcatcgtta acaacaaaaa
      601 aaccatcccg aacaacctgg ttgaaaacta cctgaccccg atgtctctgg catactggtt
      661 catggatgat ggtggtaaat gggattacaa caaaaactct accaacaaat cgatcgtact
      721 gaacacccag tctttcactt tcgaagaagt agaatacctg gttaagggtc tgcgtaacaa
      781 attccaactg aactgttacg taaaaatcaa caaaaacaaa ccgatcatct acatcgattc
      841 tatgtcttac ctgatcttct acaacctgat caaaccgtac ctgatcccgc agatgatgta
//
