LOCUS       sequence-63112-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Bennardo N, Cheng A, Huang N, Stark JM
  TITLE     Alternative-NHEJ is a mechanistically distinct pathway of mammalian 
            chromosome break repair.
  JOURNAL   PLoS Genet. 2008 Jun 27;4(6):e1000110. doi: 
            10.1371/journal.pgen.1000110.
  PUBMED    18584027
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2008-06-27"; volume: "4(6):e1000110. doi"; pages: " 
            10.1371/journal.pgen.1000110"
COMMENT     Sequence Label: EJ2GFP-puro
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(260..280)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(302..322)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(384..403)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(465..487)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 accatggtgg cgaccggtgg atccacgccg ggcttgtcat ctaattaccc tgttatccct
       61 aggcgggctt ggcgaagtgc ctcagctcca ggtgcctcct caggttcctg ctctgcctga
      121 tcacggcgta gcagatgggg caggtggcgg gctgctcgct ctggctcctg ctcctgggct
      181 tctcggtgcc gggggggtgc ttggccctct tggccttggg ggtgctcagc acgccgctgg
      241 tgttgcctcg tgggtcttct acttttcgtt ttttttttgg tggtgctgca tgcttcttcg
      301 gcacctttct cttctttttt ggcgatcttg atcccatagt ggcggcggat ctgaattaat
      361 tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa
      421 aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag
      481 ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga
      541 ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt
      601 tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc
      661 ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac
      721 gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg
      781 gg
//
