LOCUS       sequence-63113-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Bennardo N, Cheng A, Huang N, Stark JM
  TITLE     Alternative-NHEJ is a mechanistically distinct pathway of mammalian 
            chromosome break repair.
  JOURNAL   PLoS Genet. 2008 Jun 27;4(6):e1000110. doi: 
            10.1371/journal.pgen.1000110.
  PUBMED    18584027
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2008-06-27"; volume: "4(6):e1000110. doi"; pages: " 
            10.1371/journal.pgen.1000110"
COMMENT     Sequence Label: pimEJ5GFP
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(775..795)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc attaccctgt tatccctaag
       61 cttctgatgg aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga
      121 ggctgctgat ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag
      181 aaccacaaag tgggaatcaa gagaaaaaca atgatcccac gagagatcta tagatctata
      241 gatcatgagt gggaggaatg agctggccct taatttggtt ttgcttgttt aaattatgat
      301 atccaactat gaaacattat cataaagcaa tagtaaagag ccttcagtaa agagcaggca
      361 tttatctaat cccaccccac ccccaccccc gtagctccaa tccttccatt caaaatgtag
      421 gtactctgtt ctcacccttc ttaacaaagt atgacaggaa aaacttccat tttagtggac
      481 atctttattg tttaatagat catcaatttc gatccgctcc tgggcaccga actgcgccgc
      541 gtgttcagca gggtcggcgt gttcggtgtg tcccccgcgg tgggcctcgg gggcgggtgc
      601 ggggtcggcg gggccgcccc gggtggcttc ggtcggagcc atggggtcgt gcgctccttt
      661 cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg gtcatgcacc
      721 aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt
      781 agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg
      841 ccgcctccac tccgggg
//
