LOCUS       sequence-63114-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ909
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(172..189)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(172..188)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(194..205)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc tcgaggaatt
       61 cctgcaggat atctggatcc acgaagcttc ccatggtgac gtcaccggtt ctagatacct
      121 aggtgagctc tggtaccctc tagtcaaggc cttaagtgag tcgtattacg gactggccgt
      181 cgttttacat ggcacgccct tctattacag ggtttctgat aacaaaccgg ttattaactc
      241 ccaacaaggg atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca
      301 aattcatcgc ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt
      361 ctgcacccta tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt
      421 ctccccagct ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct
      481 ctctgacgtc ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt
      541 ttcccatttc tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt
      601 ttttttctgt cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc
      661 tttttcttct tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac
      721 cccgcggttc gcaataggtt tctttttcaa attattagcg ttataaatag aaaaatggtg
      781 gagtttcaat aaaaatgata atttacaaag tgtattttga ttacatgtac tcaaaaaggt
      841 tgaaattttt cataccagtt ttccggaaat ccatctga
//
