LOCUS       sequence-63137-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1208
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..607
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    633..657
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             660..680
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(811..829)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aaagttggca cctttggtct tttattgtca acttccattg gttcttccat tgtttctgtt
       61 aaattaatga atttttcata aaataaagac attatacaat ataaaaatga agaatttatt
      121 gaaaataaac tgccagagag aaaaagtatg caacactccc gccgagagtg tttgaaatgg
      181 tgtacggtac attttcgtgc taggagttag atgtgcaggc agcaacgaga gggggagaga
      241 tttttttggg ccttgtgaaa ttaacgtgag ttttctggtc atctgactaa tcatgttggt
      301 tttttgttgg tttattttgt ttttatcttt gtttttatcc agattaggaa atttaaattt
      361 tatgaattta taatgaggtc aaacattcag tcccagcgtt tttcctgttc tcactgttta
      421 gtcgaatttt tattttaggc tttcaacaaa tgttctaact gtcttatttg tgacctcact
      481 ttttatattt ttttaatttt taaaaatatt agaagtttct aggataattt tttcgacttt
      541 tattctctct accgtccgca ctcttcttac ttttaaatta aattgttttt ttttcagttg
      601 ggaaacactt tgctcactcc gtagcagcca tggcaagttt gtacaaaaaa gcaggctcgc
      661 caaagaagaa gcgtaaggtc gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct
      721 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
      781 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
      841 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
      901 ag
//
