LOCUS       sequence-63138-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1209
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..604
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    630..654
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             657..677
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(808..826)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gttggcacct ttggtctttt attgtcaact tccattggtt cttccattgt ttctgttaaa
       61 ttaatgaatt tttcataaaa taaagacatt atacaatata aaaatgaaga atttattgaa
      121 aataaactgc cagagagaaa aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt
      181 acggtacatt ttcgtgctag gagttagatg tgcaggcagc aacgagaggg ggagagattt
      241 ttttgggcct tgtgaaatta acgtgagttt tctggtcatc tgactaatca tgttggtttt
      301 ttgttggttt attttgtttt tatctttgtt tttatccaga ttaggaaatt taaattttat
      361 gaatttataa tgaggtcaaa cattcagtcc cagcgttttt cctgttctca ctgtttagtc
      421 gaatttttat tttaggcttt caacaaatgt tctaactgtc ttatttgtga cctcactttt
      481 tatatttttt taatttttaa aaatattaga agtttctagg ataatttttt cgacttttat
      541 tctctctacc gtccgcactc ttcttacttt taaattaaat tgtttttttt tcagttggga
      601 aacactttgc tcactccgta gcagccatgg caagtttgta caaaaaagca ggctcgccaa
      661 agaagaagcg taaggtcgtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
      721 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
      781 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagg
//
