LOCUS       sequence-63143-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ906
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(76..82,83..100,101..106))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(131..254)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(262..279)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(262..278)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(284..295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gacggatcct
       61 aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      121 atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
      181 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt
      241 attatacata gttgataatt cactggccgt cgttttacat ggcacgccct tctattacag
      301 ggtttctgat aacaaaccgg ttattaactc ccaacaaggg atgatttcaa ttcatcatgc
      361 tcaaattgac ccaaattaag ttacatgaca aattcatcgc ccttttcaac tcttcttggc
      421 tcatcatctg ttattctgtt cctattctgt ctgcacccta tacccctttg catactctct
      481 cgtcattcct ttcttggata gtgtcttctt ctccccagct ctgctacttc tatgacttgc
      541 gcccgctgct tttccgctcg ttcctctcct ctctgacgtc ttcttctgct tcttttctcc
      601 ttcctttttc ccttctcaat cttcctcttt ttcccatttc tgtcaatcat tcgaagaaga
      661 agaagaagaa gaccctcatt catttcattt ttttttctgt cggtgtgtgc tg
//
