LOCUS       sequence-63146-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mKalama1 blue fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE
  TITLE     Exploration of New Chromophore Structures Leads to the 
            Identification of Improved Blue Fluorescent Proteins.
  JOURNAL   Biochemistry. 2007 May 22;46(20):5904-10. doi: 10.1021/bi700199g
  PUBMED    17444659
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2007-05-22- 22"; volume: "46"; issue: "20"; 
            pages: "5904-10"
COMMENT     Sequence Label: pBad-mKalama1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(10..723,724..726,727..729))
                     /codon_start=1
                     /product="photostable monomeric blue variant of GFP (Ai et 
                     al., 2007)"
                     /label=mKalama1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVEMDGDVNGRKFSVRGVGEGDATHGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GSYKTRAEVKFEGDTLVNRIVLKGTDFKEDGNILGHKLEYNMNVGNVYITADKQKNGIK
                     ANFEIRHNVEDGGVQLADHYQQNTPIGDGSVLLPDNHYLSVQVKLSKDPNEKRDHMVLL
                     EFRTAAGITPGMDELYK"
     CDS             complement(736..756)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(769..801)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(805..822)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgaattct tacttgtaca gctcgtccat gccgggagtg atcccggcgg cggtgcggaa
       61 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tcacctgcac
      121 gctcaggtag tggttgtcgg gcagcagcac ggagccgtcg ccgatggggg tgttctgctg
      181 gtagtggtcg gcgagctgca cgccgccgtc ctcgacgttg tgcctgatct cgaagttcgc
      241 tttgatgccg ttcttctgct tgtcggccgt gatatagacg ttcccgacgt tcatgttgta
      301 ctccagcttg tgccccagga tgttaccgtc ctccttgaag tcggtgccct tcagcacgat
      361 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agctgccgtc
      421 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      481 gtcgtgctgc ttcatgtggt cggggtagcg ggagaagcac tgcacgccgt aagacagggt
      541 ggtcacgagg gtaggccagg gcacgggcag cttgccggag gtgcagatga acttcagggt
      601 cagcttgccg tgggtggcat caccctcgcc cacgccgcgc acgctgaact tgcggccgtt
      661 tacgtcgccg tccatctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
      721 gctcaccatg ctcgagccct ggaagtacag gttctcgtac agatcccgac ccatttgctg
      781 tccaccagtc atgctagcca taccatgatg atgatgatga tgagaacccc gcatat
//
