LOCUS       sequence-63148-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mKalama1 blue fluorescent protein with NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE
  TITLE     Exploration of New Chromophore Structures Leads to the 
            Identification of Improved Blue Fluorescent Proteins.
  JOURNAL   Biochemistry. 2007 May 22;46(20):5904-10. doi: 10.1021/bi700199g
  PUBMED    17444659
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2007-05-22- 22"; volume: "46"; issue: "20"; 
            pages: "5904-10"
COMMENT     Sequence Label: pmKalama1-Nuc
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..152)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(156..176)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(180..200)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc ctacctttct cttctttttt ggatctacct ttctcttctt ttttggatct
      181 acctttctct tcttttttgg atcagctcga gatctgagtc cggacttgta cagctcgtcc
      241 atgccgggag tgatcccggc ggcggtgcgg aactccagca ggaccatgtg atcgcgcttc
      301 tcgttggggt ctttgctcag cttcacctgc acgctcaggt agtggttgtc gggcagcagc
      361 acggagccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg
      421 tcctcgacgt tgtgcctggt ctcgaagttc gctttgatgc cgttcttctg cttgtcggcc
      481 gtgatataga cgttcccgac gttcatgttg tactccagct tgtgccccag gatgttaccg
      541 tcctccttga agtcggtgcc cttcagcacg atgcggttca ccagggtgtc gccctcgaac
      601 ttcacctcgg cgcgggtctt gtagctgccg tcgtccttga agaagatggt gcgctcctgg
      661 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      721 cgggagaagc actgcacgcc gtaagacagg gtggtcacga gggtaggcca gggcacgggc
      781 agcttgccgg aagtgcagat gaacttcagg gtcagcttgc cgtgggtggc atca
//
