LOCUS       sequence-63149-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1001
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(145..268)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(276..293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(276..292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(298..309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
      181 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      241 tctcgttcaa ctttattata catagttgat aattcactgg ccgtcgtttt acatggcacg
      301 cccttctatt acagggtttc tgataacaaa ccggttatta actcccaaca agggatgatt
      361 tcaattcatc atgctcaaat tgacccaaat taagttacat gacaaattca tcgccctttt
      421 caactcttct tggctcatca tctgttattc tgttcctatt ctgtctgcac cctatacccc
      481 tttgcatact ctctcgtcat tcctttcttg gatagtgtct tcttctcccc agctctgcta
      541 cttctatgac ttgcgcccgc tgcttttccg ctcgttcctc tcctctctga cgtcttcttc
      601 tgcttctttt ctccttcctt tttcccttct caatcttcct ctttttccca tttctgtcaa
      661 tcattcgaag aagaagaaga agaagaccct cattcatttc attttttttt ctgtcggtgt
      721 gtgctgctcg gttaagagtg agctctctat tccacgtctt cttctttttc ttcttcgatt
      781 cgaatcaatc actccaccaa aacgcattcg tttt
//
