LOCUS       sequence-63165-e        6388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Buck CB, Thompson CD
  TITLE     Production of papillomavirus-based gene transfer vectors.
  JOURNAL   Curr Protoc Cell Biol. 2007 Dec;Chapter 26:Unit 26.1. doi: 
            10.1002/0471143030.cb2601s37.
  PUBMED    18228512
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Cell Biol."; date: "2007-12"; volume: "Chapter 26:Unit 26.1. doi"; 
            pages: " 10.1002/0471143030.cb2601s37"
COMMENT     Sequence Label: p16L1L2
FEATURES             Location/Qualifiers
     source          1..6388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          415..511
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2065..2186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2076..2095)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2130..2149
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2387..2598
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2611..2879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     polyA_signal    4477..5049
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      5220..5808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5709..5728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5894..5941
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        5933..5951
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tacagaggcc gaggccgcct cggcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttgggatcca
      361 ccggactcta gaggatccgg tactcgagga actgaaaaac cagaaagtta actggtaagt
      421 ttagtctttt tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa tcaaagaact
      481 gctcctcagt ggatgttgcc tttacttcta ggcctgtacg gaagtgttac ttctgctcta
      541 aaagctgcgg aattgtaccc gcggccgctc tagagccacc atgaggcaca agaggagcgc
      601 caagaggacc aagagggcca gcgccaccca gctgtacaag acctgcaagc aggccggcac
      661 ctgccccccc gacatcatcc ccaaggtgga gggcaagacc atcgccgacc agatcctgca
      721 gtacggcagc atgggcgtgt tcttcggcgg cctgggcatc ggcaccggca gcggcaccgg
      781 cggcaggacc ggctacatcc ccctgggcac caggcccccc accgccaccg acaccctggc
      841 ccccgtgagg ccccccctga ccgtggaccc cgtgggcccc agcgacccca gcatcgtgag
      901 cctggtggag gagaccagct tcatcgacgc cggcgccccc accagcgtgc ccagcatccc
      961 ccccgacgtg agcggcttca gcatcaccac cagcaccgac accacccccg ccatcctgga
     1021 catcaacaac accgtgacca ccgtgaccac ccacaacaac cccaccttca ccgaccccag
     1081 cgtgctgcag ccccccaccc ccgccgagac cggcggccac ttcaccctga gcagcagcac
     1141 catcagcacc cacaactacg aggagatccc catggacacc ttcatcgtga gcaccaaccc
     1201 caacaccgtg accagcagca cccccatccc cggcagcagg cccgtggcca ggctgggcct
     1261 gtacagcagg accacccagc aggtgaaggt ggtggacccc gccttcgtga ccacccccac
     1321 caagctgatc acctacgaca accccgccta cgagggcatc gacgtggaca acaccctgta
     1381 cttcagcagc aacgacaaca gcatcaacat cgcccccgac cccgacttcc tggacatcgt
     1441 ggccctgcac aggcccgccc tgaccagcag gaggaccggc atcaggtaca gcaggatcgg
     1501 caacaagcag accctgagga ccaggagcgg caagagcatc ggcgccaagg tgcactacta
     1561 ctacgacctg agcaccatcg accccgccga ggagatcgag ctgcagacca tcacccccag
     1621 cacctacacc accaccagcc acgccgccag ccccaccagc atcaacaacg gcctgtacga
     1681 catctacgcc gacgacttca tcaccgacac cagcaccacc cccgtgccca gcgtgcccag
     1741 caccagcctg agcggctaca tccccgccaa caccaccatc cccttcggtg gcgcctacaa
     1801 catccccctg gtgagcggcc ccgacatccc catcaacatc accgaccagg cccccagcct
     1861 gatccccatc gtgcccggca gcccccagta caccatcatc gccgacgccg gcgacttcta
     1921 cctgcacccc agctactaca tgctgaggaa gaggaggaag aggctgccct acttcttcag
     1981 cgacgtgagc ctggccgcct gaaagctttt tgaattcttt ggatccacta gtgtcgacta
     2041 gagggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     2101 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2161 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2221 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     2281 tcgataagga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2341 agttgcggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
     2401 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     2461 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2521 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     2581 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     2641 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     2701 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     2761 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     2821 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     2881 aagctgtgac cggcccgctc tagagccacc atgagcctgt ggctgcccag cgaggccacc
     2941 gtgtacctgc cccccgtgcc cgtgagcaag gtggtgagca ccgacgagta cgtggccagg
     3001 accaacatct actaccacgc cggcaccagc aggctgctgg ccgtgggcca cccctacttc
     3061 cccatcaaga agcccaacaa caacaagatc ctggtgccca aggtgagcgg cctgcagtac
     3121 agggtgttca ggatccacct gcccgacccc aacaagttcg gcttccccga caccagcttc
     3181 tacaaccccg acacccagag gctggtgtgg gcctgcgtgg gcgtggaggt gggcaggggc
     3241 cagcccctgg gcgtgggcat cagcggccac cccctgctga acaagctgga cgacaccgag
     3301 aacgccagcg cctacgccgc caacgccggc gtggacaaca gggagtgcat cagcatggac
     3361 tacaagcaga cccagctgtg cctgatcggc tgcaagcccc ccatcggcga gcactggggc
     3421 aagggcagcc cctgcaccaa cgtggccgtg aaccccggcg actgcccccc cctggagctg
     3481 atcaacaccg tgatccagga cggcgacatg gtggacaccg gcttcggcgc catggacttc
     3541 accaccctgc aggccaacaa gagcgaggtg cccctggaca tctgcaccag catctgcaag
     3601 taccccgact acatcaagat ggtgagcgag ccctacggcg acagcctgtt cttctacctg
     3661 aggagggagc agatgttcgt gaggcacctg ttcaacaggg ccggcgccgt gggcgagaac
     3721 gtgcccgacg acctgtacat caagggcagc ggcagcaccg ccaacctggc cagcagcaac
     3781 tacttcccca cccccagcgg cagcatggtg accagcgacg cccagatctt caacaagccc
     3841 tactggctgc agagggccca gggccacaac aacggcatct gctggggcaa ccagctgttc
     3901 gtgaccgtgg tggacaccac caggagcacc aacatgagcc tgtgcgccgc catcagcacc
     3961 agcgagacca cctacaagaa caccaacttc aaggagtacc tgaggcacgg cgaggagtac
     4021 gacctgcagt tcatcttcca gctgtgcaag atcaccctga ccgccgacgt gatgacctac
     4081 atccacagca tgaacagcac catcctggag gactggaact tcggcctgca gccccccccc
     4141 ggcggcaccc tggaggacac ctacaggttc gtgaccagcc aggccatcgc ctgccagaag
     4201 cacacccccc ccgcccccaa ggaggacccc ctgaagaagt acaccttctg ggaggtgaac
     4261 ctgaaggaga agttcagcgc cgacctggac cagttccccc tgggcaggaa gttcctgctg
     4321 caggccggcc tgaaggccaa gcccaagttc accctgggca agaggaaggc cacccccacc
     4381 accagcagca ccagcaccac cgccaagagg aagaagagga agctgtgaaa gctacccacg
     4441 gccgaatagc cgtgagccgg aatcctgcac gctagcatta tccctaatac ctgccacccc
     4501 actcttaatc agtggtggaa gaacggtctc agaactgttt gtttcaattg gccatttaag
     4561 tttagtagta aaagactggt taatgataac aatgcatcgt aaaaccttca gaaggaaagg
     4621 agaatgtttt gtggaccact ttggttttct tttttgcgtg tggcagtttt aagttattag
     4681 tttttaaaat cagtactttt taatggaaac aacttgacca aaaatttgtc acagaatttt
     4741 gagacccatt aaaaaagtta aatgagaaac ctgtgtgttc ctttggtcaa caccgagaca
     4801 tttaggtgaa agacatctaa ttctggtttt acgaatctgg aaacttcttg aaaatgtaat
     4861 tcttgagtta acacttctgg gtggagaata gggttgtttt ccccccacat aattggaagg
     4921 ggaaggaata tcatttaaag ctatgggagg gtttctttga ttacaacact ggagagaaat
     4981 gcagcatgtt gctgattgcc tgtcactaaa acaggccaaa aactgagtcc ttgggttgca
     5041 tagaaagctt catgttgcta aaccaatgtt aagtgaatct ttggaaacaa aatgtttcca
     5101 aattactggg atgtgcatgt tgaaacgtgg gttaattaac tagccatgac caaaatccct
     5161 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5221 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5281 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5341 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5401 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5461 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5521 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5581 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5641 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5701 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5761 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5821 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt cttaattaaa
     5881 tttttcaaaa gtagttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     5941 ctcactatag gagggccatc atggccaagt tgaccagtgc tgtcccagtg ctcacagcca
     6001 gggatgtggc tggagctgtt gagttctgga ctgacaggtt ggggttctcc agagattttg
     6061 tggaggatga ctttgcaggt gtggtcagag atgatgtcac cctgttcatc tcagcagtcc
     6121 aggaccaggt ggtgcctgac aacaccctgg cttgggtgtg ggtgagagga ctggatgagc
     6181 tgtatgctga gtggagtgag gtggtctcca ccaacttcag ggatgccagt ggccctgcca
     6241 tgacagagat tggagagcag ccctggggga gagagtttgc cctgagagac ccagcaggca
     6301 actgtgtgca ctttgtggca gaggagcagg actgaggata agaattgtaa caaaaaaccc
     6361 cgccccggcg gggttttttg ttaattaa
//
