LOCUS       sequence-63175-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 May;9(5):811-23.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2010-05"; volume: "9(5)"; pages: "811-23"
COMMENT     Sequence Label: pLD-hygro-EnVM
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             342..647
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     526..545
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 acgcatggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
       61 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
      121 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
      181 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
      241 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
      301 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
      361 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
      421 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
      481 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
      541 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
      601 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
      661 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
      721 tatgtagtct gttttt
//
