LOCUS       sequence-63177-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Leterrier C, Vacher H, Fache MP, d'Ortoli SA, Castets F, 
            Autillo-Touati A, Dargent B
  TITLE     End-binding proteins EB3 and EB1 link microtubules to ankyrin G in 
            the axon initial segment.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 24;108(21):8826-31. doi: 
            10.1073/pnas.1018671108. Epub 2011 May 6.
  PUBMED    21551097
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1018671108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-24- 24"; volume: "108"; issue: "21"; pages: "8826-31"
COMMENT     Sequence Label: ankG-mCherry
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..849)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(417..436)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     686..704
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      846..855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctgcataa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
       61 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      121 tctagagtcg cggccgccct acttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      181 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
      241 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      301 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
      361 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
      421 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      481 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
      541 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
      601 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
      661 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
      721 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
      781 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
      841 gctcaccatg gtggcgaccg gtggatcccg ggcccgcggt accgtcgact ggtgggcttt
      901 cttctccaca ttccggattt ccttctttgt tttcacctta tagccttctc c
//
