LOCUS       sequence-63180-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Cheeseman IM, Desai A
  TITLE     A combined approach for the localization and tandem affinity 
            purification of protein complexes from metazoans.
  JOURNAL   Sci STKE. 2005 Jan 11;2005(266):pl1.
  PUBMED    15644491
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci STKE.";
            date: "2005-01-11"; volume: "2005(266)"; pages: "pl1"
COMMENT     Sequence Label: pIC242
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             103..147
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ctcggcatgg acgagctgta caagtccgga ggggatcggt ggagctccac cggtggcggc
       61 cgctctagag agaatcttta ttttcagggc gccgccaaat tcaaagaaac cgctgctgct
      121 aaattcgaac gccagcacat ggacagcgga ggtggaggta ctagtctcga gatgagcttc
      181 ctcctcagca gccgctcttc taaaacattc aaaccaagga agaatatccc tgaaggatct
      241 catcagtatg aactcttaaa acatgcagaa gcaactctag gaagcgggaa tctgagacaa
      301 gctgttatgt tgcctgaggg agaggatctc aatgaatgga ttgctgtgaa cactgtggat
      361 ttctttaacc agatcaacat gttatatgga actattacag aattttgcac tgaagcaagc
      421 tgtccagtca tgtctgcagg tccgagatat gaatatcact gggcagatgg tactaatatt
      481 aaaaagccaa tcaaatgttc tgcaccaaaa tacattgact atttgatgac ttgggttcaa
      541 gatcagcttg atgatgaaac tctttttcct tctaagattg gtgtcccatt tcccaaaaac
      601 tttatgtctg tggcaaagac tattctaaag cgtctgttca gggtttatgc ccatatttat
      661 caccagcact ttgattctgt gatgcagctg caagaggagg cccacctcaa cacctccttt
      721 aagcacttta ttttctttgt tcaggagttt aatctgattg ataggcgtga gctggcacct
      781 cttcaagaat taatagagaa acttaggtct aaagacagat aaaagcttcg aattctgcag
      841 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
      901 ccacatttgt agaggtttta cttgctttaa aaaac
//
