LOCUS       sequence-63183-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Cheeseman IM, Desai A
  TITLE     A combined approach for the localization and tandem affinity 
            purification of protein complexes from metazoans.
  JOURNAL   Sci STKE. 2005 Jan 11;2005(266):pl1.
  PUBMED    15644491
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci STKE.";
            date: "2005-01-11"; volume: "2005(266)"; pages: "pl1"
COMMENT     Sequence Label: pIC26
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..76
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             89..133
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 actatacaaa tgcccggggg atcggtggag ctccaccggt ggcggccgct ctagagagaa
       61 tctttatttt cagggcgccg ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca
      121 gcacatggac agcggaggtg gaggtactag tggatcctaa ttttgccgta ttttccatat
      181 tttgttttgt atatttatcc actcaccccc tctctttgtc ctgtgaatga acttgtgcca
      241 aaaaagccaa aaataattta ctttttttaa agacatatta aaacttgaaa acttggaaca
      301 atacggtgcg aaagcgaaac agagacagaa aaatgaacaa agagatccgt gaaaagtgaa
      361 cgatgattta ggcttcttgt tgggcggcgt ggatgagagt gatgttgtct cctttgagaa
      421 gaattctgcc gatcttgttg cgtcccttcg tcttcatgtt cacctcctcg gcttcgtcga
      481 acacgacatt catgaactcg tcgaatccga tgatgtagcc ctcgagacgg tgggtgacat
      541 cctcgtacaa ccagatttgg acccgagtgc ggttttggag atatctgaaa tttgggaaat
      601 tgttttctac tgattagtga aaaaatatta cctgaagatg aggttcacag gctgaaccat
      661 cactttgttg agctttctcg tcgacatttt tgcgggatga tctgtaaaaa tcaatatatt
      721 ttttgaaaga cacaaatttt taagataaat atcaagaaaa ctcgatttta gcagctattt
      781 aagctcaaaa actacaattt atggctttaa aacataattt ttcacatttt acataaattt
      841 caacgaaaaa aaatgccaaa aaacttc
//
