LOCUS       sequence-63185-e        7363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pIRESN2 GFP-uvr8at
FEATURES             Location/Qualifiers
     source          1..7363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      996..1005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1041..1069)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1664..1684
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          3119..3348
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     3331..3350
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(3406..3980,3981..3983,3984..3992)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3573..3590)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3800..3819
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4015..4818
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4078..4097)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4688..4707
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4850..4867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4856..5080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5127..5146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5206..5222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5206..5222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5219..5241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5230..5246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5254..5260,5261..5278,5279..5284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5299..5320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5437..5454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5608..6196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5688..5707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6367..7158,7159..7227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6990..7009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7228..7332)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatctgc ggcctagcta gcgctaccgg actcagatct cgagctcaag
      961 cttcgaattc taagtgagta aaggacccac cggtcgccac catggaatcc tctagcaaag
     1021 gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaacg
     1081 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1141 tgaagttcat ctgcactact ggcaaactgc ctgttccttg gccaacacta gtcactactc
     1201 tgtgctatgg tgttcaatgc ttttcaagat acccggatca tatgaaacgg catgactttt
     1261 tcaagagtgc catgcccgaa ggttatgtac aggaacgtac catctacttc aaagatgacg
     1321 gcaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1381 agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa ctcgagtaca
     1441 actataactc acacaatgta tacatcacgg cagacaaaca aaagaatggg atcaaagcta
     1501 acttcaagac ccgccacaac attgaagatg gaagcgttca actagcagac cattatcaac
     1561 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1621 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1681 taacagctgc tgggattaca catggcatgg atgaactgta taagggatcc gcggaggata
     1741 tggctgccga cgaagttacg gctcctcctc gtaaggttct tatcatctcc gctggtgcta
     1801 gccactccgt cgctcttctc tctggtgaca ttgtttgttc ttggggtcga ggagaggatg
     1861 gacagttagg tcatggcgat gcagaggatc gaccttctcc gactcagctt agcgctttag
     1921 atggccacca aattgtttcc gttacctgtg gtgctgatca cactgttgct tattcacaat
     1981 caggcatgga agtctacagt tggggatggg gtgattttgg gagattaggc catggtaact
     2041 caagcgactt gtttactccg ctaccaatca aagcattgca cggtattcgg atcaagcaga
     2101 ttgcttgtgg ggatagtcat tgtttggctg tcactatgga aggagaggtc cagagttggg
     2161 gccgcaacca gaatggtcaa cttggtctgg gggacaccga agattctcta gtgcctcaga
     2221 agattcaagc ctttgaggga atacgaatca aaatggttgc tgctggtgca gaacacactg
     2281 ctgcagttac agaagatggt gacctctatg gatggggctg gggaagatac ggaaatttgg
     2341 gattaggtga ccggactgac cgcttagttc ctgaaagagt tacctctact ggtggtgaga
     2401 aaatgtcaat ggttgcttgt ggatggcggc acacaatatc agtttcctac tctggagcat
     2461 tgtatactta tggatggagc aaatatggac agctaggaca tggagacttg gaggatcacc
     2521 ttattcctca caaactggaa gcactgagca acagttttat ctcccagatt tcgggaggtt
     2581 ggagacatac aatggcattg acttcagatg gaaaactata tggatggggt tggaataagt
     2641 ttggacaagt aggagtcggc aataatttag atcagtgttc tcctgtgcaa gtgcgatttc
     2701 ccgatgatca gaaagtagtt caagtctcat gtggatggag acataccttg gctgtcactg
     2761 aaagaaataa cgtgtttgct tggggtagag gtacaaatgg acagctcggc attggagagt
     2821 cggttgacag gaactttccc aagattatag aggcactcag cgtcgatgga gcaagtggac
     2881 aacatataga atcttctaat atcgatccat cttcagggaa aagctgggtg tcgcctgcag
     2941 agagatatgc agttgttcct gatgaaacgg gcctaacgga tggttcaagc aaaggtaatg
     3001 gaggtgatat cagtgttcca caaactgatg tcaagcgtgt acgaatttaa gcggccgcat
     3061 agataactga tccagtgtgc tggaattaat tcgctgtctg cgagggccag ctgttggggt
     3121 gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac
     3181 gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc
     3241 tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg
     3301 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
     3361 aggtccaact gcaggtcgag catgcatcta gggcggccaa ttccgcccct ctccctcccc
     3421 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     3481 gtgattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     3541 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     3601 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     3661 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     3721 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     3781 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     3841 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     3901 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     3961 ttgaaaaaca cgatgataag cttgccacaa cccgggataa ttcctgcagc caatatggga
     4021 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4081 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4141 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4201 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4261 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4321 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4381 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4441 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4501 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     4561 gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4621 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4681 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     4741 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4801 cttgacgagt tcttctgagg ggatcaattc tctagagctc gctgatcagc ctcgactgtg
     4861 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     4921 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     4981 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5041 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5101 agctggggct cgagtgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5161 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     5221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5341 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5401 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5461 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5521 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     5821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7321 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
