LOCUS       sequence-63190-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Phong C, Markson JS, Wilhoite CM, Rust MJ
  TITLE     Robust and tunable circadian rhythms from differentially sensitive 
            catalytic domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1124-9. doi: 
            10.1073/pnas.1212113110. Epub 2012 Dec 31.
  PUBMED    23277568
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1212113110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-15- 15"; volume: "110"; issue: "3"; pages: "1124-9"
COMMENT     Sequence Label: KaiC
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..104
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(108..116,117..131)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             141..164
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gcggggttct catcatcatc
       61 atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
      121 atgacgataa ggatccggga ctggaagttc tgttccaggg gcccatgact tccgctgaga
      181 tgactagccc taataataat tctgagcacc aagccatcgc taagatgcgc acgatgattg
      241 aaggctttga tgatattagt catggcggtc ttccaatcgg gcgatcgacc ctcgttagtg
      301 gtacttcagg aaccggcaag accctttttt ctattcaatt tctctataac ggtattatcg
      361 agtttgatga gcctggggtt ttcgttactt tcgaagaaac cccgcaagat atcattaaaa
      421 acgcccgtag ttttggctgg gatttagcca agctggtcga tgagggcaaa ctatttattc
      481 ttgatgcttc acccgatcca gaaggtcaag aggttgttgg cggcttcgat ctctctgctc
      541 tgattgagcg gattaattat gcaattcaaa agtatcgagc gcggcgggtt tcaattgact
      601 cggtcacgtc cgttttccag caatatgatg cctcttctgt ggttcgccgc gaactctttc
      661 ggttggtagc tcgcctaaaa caaattgggg caactacggt catgaccacc gagcgtatcg
      721 aggaatatgg cccgatcgct cgttacggtg ttgaggaatt tgtctccgat aacgtcgtga
      781 ttctccgcaa cgttttggaa ggggagcgcc gtcgccgcac cctcgaaatc ctcaagctac
      841 gtggcaccag ccacatgaaa ggggaatatc cgttcacgat tacggatcat ggcatcaata
      901 tcttcccgct cggggcaatg cgccttacgc agcgatcgtc gaacgtgcgt gtttcatctg
      961 gtgtcgtccg actcgatgaa a
//
