LOCUS       sequence-63198-e        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_PHC3
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..56
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             143..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(493..517)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(529..549)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        534..863
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      714..849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(873..892)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gcggatcagt gtggtggtcg taggaattca tggactacaa agacgatgac gacaagcaat
       61 tgacaagttt gtacaaaaaa gttggcatgg atactgaacc aaacccggga acatcttctg
      121 tgtcaacaac aaccagcagt accaccacca ccaccatcac cacttcctcc tctcgaatgc
      181 agcagccaca gatctctgtc tacagtggtt cagaccgaca tgctgtacag gcattgcatc
      241 ggccccccag ctcagctgct cagtaccttc agcaaatgta tgcagcccaa caacagcact
      301 tgatgctgca tactgcagct cttcagcagc agcatttaag cagctcccag cttcagagcc
      361 ttgctgctgt tcaggcaagt ttgtccagtg gaagaccatc tacatctccc acaggaagtg
      421 tcacacagca gtcaagtatg tcccaaacgt ctgtaagttc cctaaatttt tttttcctgg
      481 atttaaaatt ttacccaact ttcttgtaca aagtggtctc gaccctgtgg aatgtgtgtc
      541 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      601 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      661 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
      721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
      781 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
      841 ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc acggtgcgcc
      901 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgcngcg ttcgccgact
      961 accccgccac gcgccanacc gtcgatccgg aacggccaca tcgagcgggt cacgagctgc
     1021 aaaaactttt cntcangcgc gtcgggnnga natcggcaag gng
//
