LOCUS       sequence-63205-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-EF1a-CHER-WPRE
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..725
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(160..178)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     428..447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aggatcccgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
       61 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      361 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      481 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      541 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      601 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      721 agtaagaatt cgaagcttga tccggctgct aacaaagccc gacgtacgac gcgtgctagc
      781 atgcctaggt cgacccggga tccgcggact agtataatca acctctggat ta
//
