LOCUS       sequence-63218-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Galang CK, Garcia-Ramirez J, Solski PA, Westwick JK, Der CJ, 
            Neznanov NN, Oshima RG, Hauser CA
  TITLE     Oncogenic Neu/ErbB-2 increases ets, AP-1, and NF-kappaB-dependent 
            gene expression, and inhibiting ets activation blocks Neu-mediated 
            cellular transformation.
  JOURNAL   J Biol Chem. 1996 Apr 5;271(14):7992-8.
  PUBMED    8626480
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-04-5"; volume: "271(14)"; pages: "7992-8"
COMMENT     Sequence Label: FNE2-A72
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             101..121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatggact acaaggacga cgacgataag ccaaagaaaa agcgaaaggt
      121 cggatccacc atgaatgact ttggaatcaa gaacatggac caagtggccc ctgtcgccaa
      181 cagttttcgt gggacactca agcgccagcc agcctttgac accttcgatg gctctctgtt
      241 tgctgtgctc ccttctctca gtgaagatca gacactccaa gaagtgccca cgggcctgga
      301 ttctgtctcc catgactcgg ccagctgcga gctgcctttg ctcgctccct gcagcaaggc
      361 agtgatgagc caagccttaa aagccacctt cagtggcttc caaaaggagc aacgacgtct
      421 tggcatcccc aaaaacccct ggctgtggag cgagcagcag gtgtgccagt ggcttctctg
      481 ggccaccaac gagttcagcc tggtgaatgt gaacctgcac cagtttggca tgaacggcca
      541 gatgctgtgt aacctcggca aggagcgctt cctggagctg gcgcctgact ttgtgggtga
      601 catcctctgg gaacatctag agcagatgat caaagagaac caagaaaaga cagaagacca
      661 atatgaggaa aactctcacc tcaacgcggt tcctcattgg atcaacagca atacattagg
      721 cttcagcatg gaacaggctc catatggaat gcaggcacca aactacccca aagacaatct
      781 cctggacagc atgtgcccgc catcggccac gcctgcagct ctgggctctg agctccagat
      841 gttgcccaag tctcggctca acaccgtcaa tgtcaattac tgttccatca gccaggactt
      901 ccccagcagc aacgtga
//
