LOCUS       sequence-63222-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-NRLL-tdTOM
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..90)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(265..284)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     534..552
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      682..691
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(698..712)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 catgccccag gaacaggtgg tggcggccct cggagcgctc gtactgttcc acgatggtgt
       61 agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag ccgggcagtt
      121 gcacgggctt cttggccatg tagatggtct tgaactccac caggtagtgg ccgccgtcct
      181 tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct
      241 cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg ggggggaagt
      301 tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag gagtcctggg
      361 tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
      421 ggaaggacag cttcttgtaa tcggggatgt cggcggggtg cttcacgtac gccttggagc
      481 cgtacatgaa ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
      541 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
      601 cgaactcgtg gccgttcatg gagccctcca tgcgcacctt gaagcgcatg aactctttga
      661 tgacctcctc gcccttgctc accatggtgg cgggatcctt atcgtcatcg tcgtacaggt
      721 ttaaactatc gatcccgggt cgaacgctag cggcgaccgg tggatccgat gtaatggcag
      781 agatgacgta gtcactgtca gaaccagaaa ggcacagct
//
