LOCUS       sequence-63224-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pBabe_puro_DEST_Flag_FNDC3B
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..58
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(291..315)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(327..347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        332..661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      512..647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(671..690)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cggcggatca gtgtggtggt cgtaggaatt catggactac aaagacgatg acgacaagca
       61 attgacaagt ttgtacaaaa aaggtggcat gatgatgacc gaccaaatcc ctctggaact
      121 gccaccattg ctgaacggag aggtagccat gatgccccac ttggtgaatg gagatgcagc
      181 tcagcaggtt attctcgttc aagttaatcc aggtgagact ttcacaataa gagcagagga
      241 tggaacactt cagtgcattc aagatgaagt ggtgaagaga gcctgcgatt gcccaacttt
      301 cttgtacaaa gtggtctcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      361 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      421 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      481 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
      541 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      661 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
      721 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
      781 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
      841 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg ncggtctgga
      901 ccacgccgga aancgtcnaa ncgggggcgg tgtncgccga aatcggcccg cgcatggccg
      961 aattgancgg ttncccggct ggncgcncan caccanatgg aaggcttcct ggngccncac
     1021 cggcccaagg ancccncnnn ggttcctggc
//
