LOCUS       sequence-63225-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-IRBP156-tdTOM
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    82..138
                     /label=MCS
                     /note="multiple cloning site"
     CDS             450..464
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(610..628)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggg
       61 cggccgcgcg ctaccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
      121 accgcgggcc cgggatccct acacagacat ggctcagcaa gatgagatgc aatgttcctt
      181 ccgggtcaaa ccaccctggc ctcttcttca gagtccagct catgtgcttg aattagacag
      241 gattaaaggc ttagcagagc tggaagcctc acatctaact cccacattga gccccagacc
      301 ttctgtctgc ctgctaagaa gggcaaggga gagaggcagc tgcatgacca cagccttgta
      361 cacacggagc tcttatccag gagccaaggc tctgggatcc accggtcgcc gctagcgttc
      421 gacccgggat cgatagttta aacctgtacg acgatgacga taaggatccc gccaccatgg
      481 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
      541 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      601 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      661 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
      721 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      781 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
      841 aggtgaagat gcgcggcacc
//
