LOCUS       sequence-63231-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Gupta A, Hall VL, Kok FO, Shin M, McNulty JC, Lawson ND, Wolfe SA
  TITLE     Targeted Chromosomal Deletions and Inversions in Zebrafish.
  JOURNAL   Genome Res. 2013 Mar 11.
  PUBMED    23478401
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2013 Mar 11."
COMMENT     Sequence Label: pJDS70-GG-SNI
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..163
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             170..190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag caccatggac tacaaagacc atgacggtga
      121 ttataaagat catgacatcg attacaagga tgacgatgac aagatggccc ccaagaagaa
      181 gaggaaggtg ggcattcacc gcggggtacc tatggtggac ttgaggacac tcggttattc
      241 gcaacagcaa caggagaaaa tcaagcctaa ggtcaggagc accgtcgcgc aacaccacga
      301 ggcgcttgtg gggcatggct tcactcatgc gcatattgtc gcgctttcac agcaccctgc
      361 ggcgcttggg acggtggctg tcaaatacca agatatgatt gcggccctgc ccgaagccac
      421 gcacgaggca attgtagggg tcggtaaaca gtggtcggga gcgcgagcac ttgaggcgct
      481 gctgactgtg gcgggtgagc ttagggggcc tccgctccag ctcgacaccg ggcagctgct
      541 gaagatcgcg aagagagggg gagtaacagc ggtagaggca gtgcacgcct ggcgcaatgc
      601 gctcaccggg gcccccttgc ccctggagac gattaatcac cggtcgtctc cctatcgctt
      661 ctaacatcgg aggacggcca gccttggagt ccatcgtagc ccaattgtcc aggcccgatc
      721 ccgcgttggc tgcgttaacg aatgaccatc tggtggcgtt ggcatgtctt ggtggacgac
      781 ccgcgctcga tgcagtcaaa aagggtctgc ctcatgctcc cgcattgatc aaaagaacca
      841 accggcggat tcccgagaga acttcccatc gagtcgcggg atcccaacta gtcaaaagtg
      901 aactggag
//
