LOCUS       sequence-63235-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   White EA, Kramer RE, Tan MJ, Hayes SD, Harper JW, Howley PM
  TITLE     Comprehensive analysis of host cellular interactions with human 
            papillomavirus E6 proteins identifies new E6 binding partners and 
            reflects viral diversity.
  JOURNAL   J Virol. 2012 Dec;86(24):13174-86. doi: 10.1128/JVI.02172-12. Epub 
            2012 Sep 26.
  PUBMED    23015706
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02172-12"; journalName: "J Virol"; date: "2012-12"; 
            volume: "86"; issue: "24"; pages: "13174-86"
COMMENT     Sequence Label: p6659 MSCV-IP N FlagHA HPV16E6
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             86..112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    128..152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(606..630)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttccgccc tcctccttct ctggcgccgg aattagatct gccaccatgg actacaagga
       61 tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg ctgctcgagg
      121 ttatcaaaca agtttgtaca aaaaagttgg ctttcaggac ccacaggagc gacccagaaa
      181 gttaccacag ttatgcacag agctgcaaac aactatacat gatataatat tagaatgtgt
      241 gtactgcaag caacagttac tgcgacgtga ggtatatgac tttgcttttc gggatttatg
      301 tatagtatat agagatggga atccatatgc tgtatgtgat aaatgtttaa agttttattc
      361 taaaattagt gagtatagac attattgtta tagtgtgtat ggaacaacat tagaacagca
      421 atacaacaaa ccgttgtgtg atttgttaat taggtgtatt aactgtcaaa agccactgtg
      481 tcctgaagaa aagcaaagac atctggacaa aaagcaaaga ttccataata taaggggtcg
      541 gtggaccggt cgatgtatgt cttgttgcag atcatcaaga acacgtagag aaacccagct
      601 gtaataccca actttcttgt acaaagtggt tcgataacga attccgcccc ccccccc
//
