LOCUS       sequence-63254-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Galang CK, Garcia-Ramirez J, Solski PA, Westwick JK, Der CJ, 
            Neznanov NN, Oshima RG, Hauser CA
  TITLE     Oncogenic Neu/ErbB-2 increases ets, AP-1, and NF-kappaB-dependent 
            gene expression, and inhibiting ets activation blocks Neu-mediated 
            cellular transformation.
  JOURNAL   J Biol Chem. 1996 Apr 5;271(14):7992-8.
  PUBMED    8626480
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-04-5"; volume: "271(14)"; pages: "7992-8"
COMMENT     Sequence Label: pCIN4-EZOD
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             172..192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcgatatc tgcggccgcg tcgacggaat tcgactcact
      121 atagggagac ccaagcttgc caccatggac tacaaggacg acgacgataa gccaaagaaa
      181 aagcgaaagg tcggatccac catggactac atccaagaga ggagcgaccc agtcgagcaa
      241 ggcaaaccag ttattcctgc agcagtactg gctggcttca ctggaagcgg accaatccag
      301 ttgtggcagt ttcttctgga gctactctct gacaagtcct gtcaatcttt catcagctgg
      361 acgggggatg gatgggagtt caagcttgct gaccccgatg aggttgcccg ccggtggggg
      421 aagaggaaaa ataaaccaaa gatgaactac gagaagctga gccggggctt acgttactac
      481 tacgacaaga acatcatcca caagacttcg ggcaagcgct acgtgtaccg tttcgtatgt
      541 gacctgcaga acttgctggg cttcactccg gaggaactgc atgccatcct gggcgtccag
      601 cctgatacag aagactgatc gacccgggcg aatccatcac actggcggcc gctcgagcat
      661 gcatctagag ggccgaattc agtggatcca ctagtaacgg ccgccagtgt gctggaatta
      721 attcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga
      781 cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg
      841 cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga aaagaca
//
