LOCUS       sequence-63256-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 May;9(5):811-23.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2010-05"; volume: "9(5)"; pages: "811-23"
COMMENT     Sequence Label: pLD-hygro-EnVM
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..56
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             66..95
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    108..232
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(257..262,263..280,281..287)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(407..426)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aggctagcac catgggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
       61 caggtgagca aaagctcatt tctgaggaag atctcaccgg taagcttaca agtttgtaca
      121 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
      181 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
      241 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
      301 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      361 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      421 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      481 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      541 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      601 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      661 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      721 cggtgaaaac ctggcctatt tccctaaa
//
