LOCUS       sequence-63259-e        9133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pIRESN2 H2B-GFP-uvr8at(2x)
FEATURES             Location/Qualifiers
     source          1..9133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             993..1370
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      1383..1392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1428..1456)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2051..2071
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          4889..5118
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5101..5120
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5176..5750,5751..5753,5754..5762)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5343..5360)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5570..5589
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5785..6588
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5848..5867)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6458..6477
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6620..6637)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6626..6850
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6897..6916
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6976..6992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6976..6992)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6989..7011)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7000..7016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7024..7030,7031..7048,7049..7054))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7069..7090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7207..7224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7378..7966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7458..7477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8137..8928,8929..8997))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8760..8779
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8998..9102)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatctgc ggcctagcta gcgctaccgg actcagatct cgagctcaag
      961 cttcgaattc tgcagtcgac ggtaccgcca ccatgccaga gccagcgaag tctgctcccg
     1021 ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc
     1081 gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc
     1141 accctgacac cggcatttcg tccaaggcta tgggcatcat gaattcgttt gtgaacgaca
     1201 ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca
     1261 tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc
     1321 acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag gacccaccgg
     1381 tcgccaccat ggaatcctct agcaaaggag aagaactctt cactggagtt gtcccaattc
     1441 ttgttgaatt agatggtgat gttaacggcc acaagttctc tgtcagtgga gagggtgaag
     1501 gtgatgcaac atacggaaaa cttaccctga agttcatctg cactactggc aaactgcctg
     1561 ttccttggcc aacactagtc actactctgt gctatggtgt tcaatgcttt tcaagatacc
     1621 cggatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1681 aacgtaccat ctacttcaaa gatgacggca actacaagac acgtgctgaa gtcaagtttg
     1741 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgacttcaag gaagatggca
     1801 acattctggg acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag
     1861 acaaacaaaa gaatgggatc aaagctaact tcaagacccg ccacaacatt gaagatggaa
     1921 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1981 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     2041 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     2101 aactgtataa gggatccgcg gaggatatgg ctgccgacga agttacggct cctcctcgta
     2161 aggttcttat catctccgct ggtgctagcc actccgtcgc tcttctctct ggtgacattg
     2221 tttgttcttg gggtcgagga gaggatggac agttaggtca tggcgatgca gaggatcgac
     2281 cttctccgac tcagcttagc gctttagatg gccaccaaat tgtttccgtt acctgtggtg
     2341 ctgatcacac tgttgcttat tcacaatcag gcatggaagt ctacagttgg ggatggggtg
     2401 attttgggag attaggccat ggtaactcaa gcgacttgtt tactccgcta ccaatcaaag
     2461 cattgcacgg tattcggatc aagcagattg cttgtgggga tagtcattgt ttggctgtca
     2521 ctatggaagg agaggtccag agttggggcc gcaaccagaa tggtcaactt ggtctggggg
     2581 acaccgaaga ttctctagtg cctcagaaga ttcaagcctt tgagggaata cgaatcaaaa
     2641 tggttgctgc tggtgcagaa cacactgctg cagttacaga agatggtgac ctctatggat
     2701 ggggctgggg aagatacgga aatttgggat taggtgaccg gactgaccgc ttagttcctg
     2761 aaagagttac ctctactggt ggtgagaaaa tgtcaatggt tgcttgtgga tggcggcaca
     2821 caatatcagt ttcctactct ggagcattgt atacttatgg atggagcaaa tatggacagc
     2881 taggacatgg agacttggag gatcacctta ttcctcacaa actggaagca ctgagcaaca
     2941 gttttatctc ccagatttcg ggaggttgga gacatacaat ggcattgact tcagatggaa
     3001 aactatatgg atggggttgg aataagtttg gacaagtagg agtcggcaat aatttagatc
     3061 agtgttctcc tgtgcaagtg cgatttcccg atgatcagaa agtagttcaa gtctcatgtg
     3121 gatggagaca taccttggct gtcactgaaa gaaataacgt gtttgcttgg ggtagaggta
     3181 caaatggaca gctcggcatt ggagagtcgg ttgacaggaa ctttcccaag attatagagg
     3241 cactcagcgt cgatggagca agtggacaac atatagaatc ttctaatatc gatccatctt
     3301 cagggaaaag ctgggtgtcg cctgcagaga gatatgcagt tgttcctgat gaaacgggcc
     3361 taacggatgg ttcaagcaaa ggtaatggag gtgatatcag tgttccacaa actgatgtca
     3421 agcgtgtacg aattggtgac tctggagacg gtaagggtgg caaaggctcc ggagatggtt
     3481 ctggtggtga cggttctggt gcggaggata tggctgccga cgaagttacg gctcctcctc
     3541 gtaaggttct tatcatctcc gctggtgcta gccactccgt cgctcttctc tctggtgaca
     3601 ttgtttgttc ttggggtcga ggagaggatg gacagttagg tcatggcgat gcagaggatc
     3661 gaccttctcc gactcagctt agcgctttag atggccacca aattgtttcc gttacctgtg
     3721 gtgctgatca cactgttgct tattcacaat caggcatgga agtctacagt tggggatggg
     3781 gtgattttgg gagattaggc catggtaact caagcgactt gtttactccg ctaccaatca
     3841 aagcattgca cggtattcgg atcaagcaga ttgcttgtgg ggatagtcat tgtttggctg
     3901 tcactatgga aggagaggtc cagagttggg gccgcaacca gaatggtcaa cttggtctgg
     3961 gggacaccga agattctcta gtgcctcaga agattcaagc ctttgaggga atacgaatca
     4021 aaatggttgc tgctggtgca gaacacactg ctgcagttac agaagatggt gacctctatg
     4081 gatggggctg gggaagatac ggaaatttgg gattaggtga ccggactgac cgcttagttc
     4141 ctgaaagagt tacctctact ggtggtgaga aaatgtcaat ggttgcttgt ggatggcggc
     4201 acacaatatc agtttcctac tctggagcat tgtatactta tggatggagc aaatatggac
     4261 agctaggaca tggagacttg gaggatcacc ttattcctca caaactggaa gcactgagca
     4321 acagttttat ctcccagatt tcgggaggtt ggagacatac aatggcattg acttcagatg
     4381 gaaaactata tggatggggt tggaataagt ttggacaagt aggagtcggc aataatttag
     4441 atcagtgttc tcctgtgcaa gtgcgatttc ccgatgatca gaaagtagtt caagtctcat
     4501 gtggatggag acataccttg gctgtcactg aaagaaataa cgtgtttgct tggggtagag
     4561 gtacaaatgg acagctcggc attggagagt cggttgacag gaactttccc aagattatag
     4621 aggcactcag cgtcgatgga gcaagtggac aacatataga atcttctaat atcgatccat
     4681 cttcagggaa aagctgggtg tcgcctgcag agagatatgc agttgttcct gatgaaacgg
     4741 gcctaacgga tggttcaagc aaaggtaatg gaggtgatat cagtgttcca caaactgatg
     4801 tcaagcgtgt acgaatttaa gcggccgcat agataactga tccagtgtgc tggaattaat
     4861 tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact
     4921 tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg
     4981 gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca
     5041 agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact
     5101 ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta
     5161 gggcggccaa ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     5221 ggaataaggc cggtgtgcgt ttgtctatat gtgattttcc accatattgc cgtcttttgg
     5281 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5341 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5401 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5461 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5521 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5581 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5641 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5701 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa
     5761 cccgggataa ttcctgcagc caatatggga tcggccattg aacaagatgg attgcacgca
     5821 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5881 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5941 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     6001 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6061 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6121 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6181 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6241 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6301 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc
     6361 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6421 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6481 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6541 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg ggatcaattc
     6601 tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6661 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6721 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6781 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6841 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgagtgcatt ctagttgtgg
     6901 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6961 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7021 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7081 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7141 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7201 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7261 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7321 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7381 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7441 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7501 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7561 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7621 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7681 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7741 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7801 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7861 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7921 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7981 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8041 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8101 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8161 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8221 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8281 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8341 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8401 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8461 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8521 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8581 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8641 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8701 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8761 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8821 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8881 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8941 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9001 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9061 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9121 gccacctgac gtc
//
