LOCUS       sequence-63262-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative selection marker for use with the Mos-1 mediated system of 
            creating targeted transgene insertions and targeted deletions in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pMA122 - peel-1 negative selection
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..333)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    338..362
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(891..915)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagttgtgag acttttttct tggcggcaca ataaagtttc ctctttttgt tgggtcactc
       61 aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg ggtttaaagc
      121 acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc acattttgat
      181 agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat aaaataataa
      241 cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag aagagtgata
      301 gagaagaagg gaatgcttga aaggattttg catttatccc actttgtaca agaaagctgg
      361 gtctcatgga ttttcaacac ttggatcaat atgagcagtt gagttgagac aattaaatcg
      421 tacagcagcg cacaaagttg gtctcaggcc agctgtttga actaattcca tcaacggatg
      481 gatcgcacca cgagactcat aataattcag cagcaaggag aacagtgcag agttcaatga
      541 taagagcata ttctggcatg ggtcaatgta aaggacatgg gcgaaaatct gtgcaagaaa
      601 tatcaagcca atcaaaccct tgcaaacaag aagcaggata gtttttgacg agacagaaaa
      661 tcttccttgc ttaatttcaa atgggctaaa aaaaattaac cacaatgaag caattgatcc
      721 aataagagca ataacatgcg caaaccgatt tacccataca ttttcaggca gatcaagata
      781 catgtagatt ttgaaaatct ttacaagagc aagtaaaaat ccgactatga tgttccacaa
      841 aaatatgaaa atcatcgaaa attttaagtt ttggaaatca aagcgcatga agcctgcttt
      901 tttgtacaaa cttgattttt t
//
