LOCUS       sequence-63265-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ420 - Peft-3::GFP::H2B (ubiquitous)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..37)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(46..645)
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    complement(646..666)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ctttactcat ggagcctgct tttttgtaca aacttgtgag caaagtgttt cccaactgaa
       61 aaaaaaacaa tttaatttaa aagtaagaag agtgcggacg gtagagagaa taaaagtcga
      121 aaaaattatc ctagaaactt ctaatatttt taaaaattaa aaaaatataa aaagtgaggt
      181 cacaaataag acagttagaa catttgttga aagcctaaaa taaaaattcg actaaacagt
      241 gagaacagga aaaacgctgg gactgaatgt ttgacctcat tataaattca taaaatttaa
      301 atttcctaat ctggataaaa acaaagataa aaacaaaata aaccaacaaa aaaccaacat
      361 gattagtcag atgaccagaa aactcacgtt aatttcacaa ggcccaaaaa aatctctccc
      421 cctctcgttg ctgcctgcac atctaactcc tagcacgaaa atgtaccgta caccatttca
      481 aacactctcg gcgggagtgt tgcatacttt ttctctctgg cagtttattt tcaataaatt
      541 cttcattttt atattgtata atgtctttat tttatgaaaa attcattaat ttaacagaaa
      601 caatggaaga accaatggaa gttgacaata aaagaccaaa ggtgccaact tttctataca
      661 aagttgatag cttggcggat ccctggcgtt acccaactta atcgccttgc agcacatccc
      721 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      781 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      841 atttcacacc gcatatggtg cactctcagt gcaatctgct ctgatgccgc atagtta
//
